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基于光谱学和电化学方法的莠去津毒理作用评价
引用本文:时伟杰,艾仕云,朱鲁生,马永飞,李金焕.基于光谱学和电化学方法的莠去津毒理作用评价[J].生态毒理学报,2008,3(2):178-182.
作者姓名:时伟杰  艾仕云  朱鲁生  马永飞  李金焕
作者单位:1. 山东农业大学化学与材料科学学院,泰安,271018;山东农业大学资源与环境学院,泰安,271018
2. 山东农业大学化学与材料科学学院,泰安,271018
3. 山东农业大学资源与环境学院,泰安,271018
基金项目:国家自然科学基金 , 山东省自然科学基金
摘    要:为探索莠去津对DNA的损伤及其毒性作用机制,采用紫外吸收光谱法、荧光光谱法以及电化学方法研究了莠去津与鲱鱼精DNA的相互作用,探讨了二者形成DNA加合物的作用方式.结果表明,莠去津与鲱鱼精DNA作用后,莠去津的紫外光谱呈现减色效应,并有轻微红移现象,而其荧光光谱强度明显增强;循环伏安法显示莠去津与DNA作用能引起莠去津还原电位正移,峰电流减小.以上实验结果表明,莠去津平面分子能够嵌插到DNA双螺旋链中,形成较稳定的加合物.采用电化学方法测得莠去津与鲱鱼精DNA的结合比为1:1,结合常数为1.2×105.该研究为莠去津毒理作用评价提供了一种简便的方法。

关 键 词:DNA  莠去津  紫外吸收光谱法  荧光光谱法  循环伏安法
文章编号:1673-5897(2008)2-178-05
收稿时间:2007/12/24 0:00:00
修稿时间:2007年12月24

Toxicological Evaluation of Atrazine Based on Spectroscopic and Voltammetric Methods
SHI Wei-jie,AI Shi-yun,ZHU Lu-sheng,MA Yong-fei,LI Jin-huan.Toxicological Evaluation of Atrazine Based on Spectroscopic and Voltammetric Methods[J].Asian Journal of Ecotoxicology,2008,3(2):178-182.
Authors:SHI Wei-jie  AI Shi-yun  ZHU Lu-sheng  MA Yong-fei  LI Jin-huan
Abstract:In order to explore the DNA damage effects induced by atrazine,ultraviolet absorption spectrometry,fluorescence spectrometry and cyclic voltammetry were used to study the interaction between atrazine and Herring Sperm DNA.The mode of action of DNA adducts formed by atrazine was also studied.Results showed that a hypochromic effect and a slight red shift on the UV spectrogram of atrazine were observed after the interaction with Herring Sperm DNA,while the fluorescence intensity increased obviously.Cyclic voltammetry indicated that the reductive potential of atrazine shifted positively,and the peak current decreased after the interaction with DNA.The above results proved that atrazine could be inserted into the double-helix of DNA and formed a stable DNA adduct.The binding ratio and binding constant between atrazine and DNA were measured by electrochemical method,and the values were 1:1 and 1.2×105,respectively.This research provided a simple method for the evaluation of the toxicological effects of atrazine.
Keywords:DNA  atrazine  ultraviolet absorption spectrometry  fluorescence spectrometry  cyclic voltammetry
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