排水管道沉积物微生物群落及环境因子分析

选取北京市某区的排水管道沉积物进行取样，采用高通量测序手段分析，结果表明变形菌门、广古菌门、拟杆菌门、厚壁菌门是排水管道沉积物微生物中的优势门类；在纲水平上，δ-变形菌纲、甲烷微菌纲、梭菌纲、拟杆菌纲占相对优势；在属水平上，功能性微生物硫酸盐还原菌（SRB）和产甲烷古菌（MA）普遍存在于各处管道.在所选的6段管段中，管段S3、S4处MA的相对丰度分别为20.6%、40.8%，高于其他管段，厌氧产甲烷的潜能较大，有发生可燃气积累的风险；管段S5、S6处的SRB相对丰度分别为9.14%、8.19%，高于其他管段，硫酸盐还原为硫化物的潜能较大，存在管道腐蚀的风险.RDA分析表明污水的DO、水温、硫酸根、TN与管道沉积物中微生物群落存在相关性.

Analysis of microbial communities and environmental factors in sewer sediments

Sewer sediments were sampled in a district of Beijing and analyzed using high-throughput sequencing, it was found that Proteobacteria, Euryarchaeota, Firmicutes, Bacteroidetes were the dominant phyla, and Deltaproteobacteria, Methanomicrobia, Clostridia, Bacteroidia were the dominant classes of all samples. The functional microorganisms Sulfate-Reducing Bacteria (SRB) and Methanogenic Archaea (MA) were prevalent in sewer sediments at the genus level. Among the six sewer pipes sampled, the relative abundances of MA were 20.6% and 40.8% in the sewer pipes S3 and S4, higher than other sewer pipes, which implied higher the methane production potential. Thus there was a risk of combustible gas accumulation in S3 and S4. The relative abundances of SRB were 9.14% and 8.19% in the sewer pipes S5 and S6, higher than other sewer pipes, which implied that the potential of sulfate reduced to sulfide was greater. Thus there was a risk of sewer corrosion in S5 and S6. Redundancy Analysis (RDA) denoted that the dissolved oxygen, temperature, sulfate, total nitrogen of sewage had correlation with the microbial communities in the sewer pipe sediments.