| 应用流式细胞分析技术研究溴氯海因对近头状伪蹄形藻的毒性作用 |
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| 引用本文： | 李万芳,史蕤,王福鑫,魏金锋,王爱平,靳洪涛.应用流式细胞分析技术研究溴氯海因对近头状伪蹄形藻的毒性作用[J].生态毒理学报,2011,6(2):219-224. |
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| 作者姓名： | 李万芳 史蕤 王福鑫 魏金锋 王爱平 靳洪涛 |
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| 作者单位： | 中国医学科学院北京协和医学院药物研究所新药安全评价研究中心，北京，100049;中国医学科学院北京协和医学院药物研究所新药安全评价研究中心，北京，100050;中国医学科学院北京协和医学院药物研究所新药安全评价研究中心，北京，100051;中国医学科学院北京协和医学院药物研究所新药安全评价研究中心，北京，100052;中国医学科学院北京协和医学院药物研究所新药安全评价研究中心，北京，100053;中国医学科学院北京协和医学院药物研究所新药安全评价研究中心，北京，100054 |
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| 基金项目： | “重大新药创制”科技重大专项(No. 2008ZX09305\|001) |
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| 摘 要： | 实验采用流式细胞术的检测分析方法,探究溴氯海因(BCDMH)对常见淡水藻类近头状伪蹄形藻的作用.研究结果显示BCDMH对近头状伪蹄形藻钓生长抑制作用呈明显的浓度依赖型变化.根据不同浓度细胞生长抑制的百分率计算,24、48和72 h BCDMH对近头状伪蹄形藻生长抑制作用的EC50分别为3.64、3.21和2.56 mg...
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| 关 键 词： | 溴氯海因(BCDMH) 近头状伪蹄形藻 流式细胞术(FCM) 酯酶 活性氧(ROS) |
| 收稿时间： | 2011/2/14 0:00:00 |
| 修稿时间： | 2011/4/12 0:00:00 |
Study on Toxic Effect of Bromo\|chloro\|dimethylhydantoin on Pseudokirchneriella subcapitata by Flow Cytometry |
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| Li Wanfang,Shi Rui,Wang Fuxin,Wei Jinfeng,Wang Aiping and Jin Hongtao.Study on Toxic Effect of Bromo\|chloro\|dimethylhydantoin on Pseudokirchneriella subcapitata by Flow Cytometry[J].Asian Journal of Ecotoxicology,2011,6(2):219-224. |
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| Authors: | Li Wanfang Shi Rui Wang Fuxin Wei Jinfeng Wang Aiping and Jin Hongtao |
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| Institution: | Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China;Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100051, China;Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100052, China;Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100053, China;Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100054, China;Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100055, China |
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| Abstract: | Flow cytometry (FCM) was applied to analyze the effect of bromo\|chloro\|dimethylhydantoin (BCDMH) on Pseudokirchneriella subcapitata. The results showed a concentration\|dependent growth inhibition effect of BCDMH on P. subcapitata. As the BCDMH concentration increased, the effect of inhibition profoundly risen. EC50 of BCDMH to P. subcapitata were 3.64 mg·L-1 (24 h), 3.21 mg·L-1 (48 h), and 2.56 mg·L-1 (72 h). No observed effect concentration (NOEC) of growth inhibition was 0.31 mg·L-1. Under the effect of growth inhibition, ROS content, esterase activity and membrane integrity varied; NOEC were 0.16 mg·L-1, 0.31 mg·L-1 and 1.3 mg·L-1, respectively. Among all of the changes, increased ROS concentration was the most sensitive index to BCDMH concentration, followed by changes in esterase activity. A slight membrane damage only appeared in 5.0 mg L-1 and 2.5 mg·L-1 groups. Based on effect of application, minimizing the concentration should be recommended. |
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| Keywords: | bromo\|chloro\|dimethyl hydantoin (BCDMH) Pseudokirchneriella subcapitata flow cytometry (FCM) esterase reactive oxygen species (ROS) |
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