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| Hg^2＋诱导发光报告基因系统的构建及其检测红壤中残汞的研究 | |
| 引用本文： | 何伟,韩诚,钟文辉,林先贵.Hg^2＋诱导发光报告基因系统的构建及其检测红壤中残汞的研究[J].环境科学,2011,32(10):3045-3052. |
| 作者姓名： | 何伟 韩诚 钟文辉 林先贵 |
| 作者单位： | 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008／南京师范大学生命科学学院,南京210046 2. 南京师范大学环境科学与工程系,南京,210046 3. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京,210008 |
| 基金项目： | 土壤与农业可持续发展国家重点实验室基金项目（0751010017）；南京师范大学“211工程”重点学科建设项目(1843203623)；江苏省普通高校自然科学研究计划资助项目（10KJD610001） |
| 摘 要： | 将汞诱导型启动子PmerT和其调控基因merR与egfp基因融合构建发光报告基因系统.通过mini-Tn5系统把merR-egfp插入到Pseudomonas putida染色体DNA上,构建成为在Hg2＋诱导下能特异性表达绿色荧光蛋白的工程菌,并将其应用于检测江西红壤中汞污染.当土壤中汞含量在0.04～50 mg.k... |
| 关 键 词： | 发光 汞 报告基因 红壤 构建 |
| 收稿时间： | 2010/11/16 0:00:00 |
| 修稿时间： | 2011/1/24 0:00:00 |
Construction of Hg2+-induced Luminescent Reporter Gene System and Its Application to Detect Mercury in Red Soil |
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| HE Wei,HAN Cheng,ZHONG Wen-hui and LIN Xian-gui.Construction of Hg2+-induced Luminescent Reporter Gene System and Its Application to Detect Mercury in Red Soil[J].Chinese Journal of Environmental Science,2011,32(10):3045-3052. | |
| Authors: | HE Wei HAN Cheng ZHONG Wen-hui and LIN Xian-gui |
| Institution: | HE Wei1,2,HAN Cheng3,ZHONG Wen-hui3,LIN Xian-gui1(1.State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China,2.College of Life Science,Nanjing Normal University,Nanjing 210046,3.Department of Environmental Science and Engineering,China) |
| Abstract: | A luminescent reporter gene system was constructed by fusing the mercury-inducible promoter,PmerT,and its regulatory gene,merR with a promoterless reporter gene EGFP.A stable whole-cell reporter was created by mini-Tn5 and introducing the merR-egfp system cassette into the chromosome of Pseudomonas putida strain,then applied it for mercury detection in the red soil of Jiangxi province.the fluorescence density of the sensor strain was confirmed in soil extraction and fluorescence intensity was quantified by ... |
| Keywords: | luminescent mercury reporter gene red soil construction |
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