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恶臭假单胞菌(Pseudomonas putida LY1)共代谢降解苯酚和4-氯苯酚系统的降解动力学研究
引用本文:李晶,饶婷,李巍,李轶.恶臭假单胞菌(Pseudomonas putida LY1)共代谢降解苯酚和4-氯苯酚系统的降解动力学研究[J].环境科学学报,2011,31(10):2109-2116.
作者姓名:李晶  饶婷  李巍  李轶
作者单位:1. 河海大学环境学院浅水湖泊综合治理与资源开发教育部重点实验室,南京210098/泰州市环境科学研究所,泰州225300
2. 河海大学环境学院浅水湖泊综合治理与资源开发教育部重点实验室,南京,210098
基金项目:国家自然科学基金项目(No.51009050)
摘    要:对恶臭假单胞菌(Pseudomonas putida LY1)共代谢降解苯酚和4-氯苯酚(4-CP)系统进行了降解实验和动力学研究.结果表明,恶臭假单胞菌可以有效地降解苯酚,苯酚浓度为50mg·L-1时细菌生长速度最快,4-氯苯酚浓度的增加会对细菌产生一定的抑制作用.同时,用改进的Haldane方程模拟恶臭假单胞菌LY...

关 键 词:共代谢降解  4-氯苯酚  苯酚  动力学  恶臭假单胞菌
收稿时间:2010/12/3 0:00:00
修稿时间:2011/1/20 0:00:00

Kinetic study on cometabolic biodegradation of phenol and 4-chlorophenol by Pseudomonas putida LY1
LI Jing,RAO Ting,LI Wei and LI Yi.Kinetic study on cometabolic biodegradation of phenol and 4-chlorophenol by Pseudomonas putida LY1[J].Acta Scientiae Circumstantiae,2011,31(10):2109-2116.
Authors:LI Jing  RAO Ting  LI Wei and LI Yi
Institution:1. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098;2. Taizhou Institute of Environmental Science, Taizhou 225300,Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098,Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098 and Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098
Abstract:Degradation of phenol and 4-chlorophenol by Pseudomonas putida LY1 was studied. It was found that phenol can be effectively degraded by the bacteria with an optimum phenol concentration at 50 mg·L-1. Inhibition of the bacteria increased with the 4-chlorophenol concentration. A modified Haldane equation was used to study the biodegradation of phenol by Pseudomonas putida LY1. The equation parameters calculated using MATLAB software were as follows: Rm=0.113 mg·mg-1·h-1, Ks =3.5 mg·L-1 and Kp =126.6 mg·L-1. The modified Haldane equation was also used to simulate the cometabolic biodegradation of phenol and 4-chlorophenol by Pseudomonas putida LY1. The cometabolic degradation model can simulate the process of cometabolic degradation very well. The model provides a theoretical and technical reference for the biodegradation of chlorinated refractory organic pollutants in wastewater treatment plants.
Keywords:cometabolic degradation  4-chlorophenol  phenol  kinetics  Pseudomonas putida LY1
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