硫丹致生精细胞凋亡及其机制研究

即将列入持久性有机污染物(persistent organic pollutants,POPs)的硫丹对生物体的影响已经成为国际关注的焦点.为探讨硫丹对生精细胞凋亡影响及机制,以7.0、3.5和1.75 mg/kg剂量灌胃,连续染毒小鼠28 d.采用激光共聚焦扫描显微镜观察到染毒组小鼠凋亡的生精细胞形态学特征,二苯胺法测定到各染毒组DNA降解率显著升高(p＜0.01).利用Fluo-3能特异性地与细胞内钙离子结合并激发产生激光的特性,采用激光共聚焦扫描显微镜观察到染毒后小鼠生精细胞内游离钙离子浓度升高(p＜0.01).紫外分光光度法检测到生精细胞Na K -ATPase及Ca2 Mg2 -ATPase活力显著下降(p＜0.01),结果硫丹可诱导睾丸生精细胞凋亡.说明硫丹可抑制Na K -ATPase及Ca2 Mg2 -ATPase活力,使细胞膜主动转运Ca2 的功能受限,细胞的排钙能力降低,导致细胞内Ca2 浓度持续升高,即细胞钙超载,继发细胞钙稳态失衡.

Induced Apoptosis and Mechanism of Endosulfan in Mouse Germ Cells

Endosulfan which is going to be included as POPs in effect of organism have become the focus of international concern.To explore endosulfan on apoptosis of spermatogenic cells and mechanisms,doses of 7.0,3.5 and 1.75(mg/kg) oral administration to mice exposed to 28 consecutive days.Morphology characters of spermatogenic cells observed by laser confocal scanning microscope(LCSM),DNA ladder detected by electricity swims,and increasing of DNA degeneration rate measured through diphenylamine(p<0.01).Endosulfan might enhance calcium concentration in the spermatogenic cells and restrained Na~ K~ -ATPase and Ca~(2 )Mg~(2 )-ATPase activities that showed endosulfan might induce spermatogenic cells in the testicle to occur apoptosis(p<0.01),to cause the function of transporting Ca~(2 ) to be limited,reduce the ability of excluding calcium and send Ca~(2 ) concentration to hoist sustainingly,i.e.calcium overload in the cells.