转聚磷激酶大肠杆菌诱导表达条件及磷浓度对其除磷能力的影响

在优化转聚磷激酶基因的大肠杆菌（BL-PPK）诱导表达条件基础上探讨了外界磷浓度对其除磷能力的影响，发现37℃细胞对数生长早期添加1．5mmol／LIPTG时聚磷激酶的表达活性和细胞除磷效率最高，同时BL—PPK对高达35mg／L的磷酸盐在6．5h内的去除效果达到99％以上，并以聚磷的形式积累在细胞内。

Effect of Induction Conditions and Phosphate Concentration on the Ability of Polyphosphate Kinase Gene Transferred E.coli to Remove Phosphate

On the basis of optimizing the induction conditions for gene transferred E.coli(BL-PPK) which can overexpress polyphosphate kinase gene,this experiment studied the effect of environmental soluble phosphate on the ability of BL-PPK to remove phosphate,and we got the conclusions: the optimal IPTG induction concentration for BL-PPK was 1.5mmol/L,the optimal initial cell density for induction was at the early log growth,the optimal induction temperature was 37℃.BL-PPK could remove above 99% of phosphates in 6.5h when the environmental phosphate concentration was 35mg/L,with polyphosphate accumulated.