| 应用植物酯酶抑制技术测定蔬菜水果中农药残留量 |
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| 引用本文： | 李治祥,翟延路.应用植物酯酶抑制技术测定蔬菜水果中农药残留量[J].环境科学学报,1987,7(4):472-478. |
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| 作者姓名： | 李治祥 翟延路 |
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| 作者单位： | 农牧渔业部环境保护研究所
(李治祥)，农牧渔业部环境保护研究所(翟延路) |
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| 摘 要： | 应用植物酯酶的抑制技术,测定蔬菜水果中有机磷和氨基甲酸酯类农药残留量的简易薄层—植物酶抑制(TLC-EI)法。该法对多种农药的最小检出量为毫微克(ng)级,最小检出浓度为0.01pm,添加回收率在80％以上。
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| 关 键 词： | 植物酯酶 农药残留 酶抑制技术 薄层层析 |
| 收稿时间： | 2/9/1987 12:00:00 AM |
DETERMINATION OF PESTICIDE RESIDUES IN VEGETABLES AND FRUITS USING A PLANT-ESTERASE INHIBITION TECHNIQUE |
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| Li Zhixiang and Zhai Yanlu.DETERMINATION OF PESTICIDE RESIDUES IN VEGETABLES AND FRUITS USING A PLANT-ESTERASE INHIBITION TECHNIQUE[J].Acta Scientiae Circumstantiae,1987,7(4):472-478. |
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| Authors: | Li Zhixiang and Zhai Yanlu |
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| Institution: | Environmental Protection Institute, Ministry of Agriculture, Animal Husbandry and Fishery and Environmental Protection Institute, Ministry of Agriculture, Animal Husbandry and Fishery |
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| Abstract: | A thin-layer chromatographic-plant enzyme inhibition (TLC-EI) technique was employed to determine the contents of carbamate and organophosphorus pesticides in vegetable and fruit extracts. Detection of nanogram amounts of these pesticides was achieved with a water extracts of flour as source of esterase and 1-naphthyl acetate as substrate. Satisfactory results were obtained when pesticide contents in vegetables and fruits were 0.0lppm and the recoveries were more than 80%. |
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| Keywords: | esterase pesticide enzymatic inhibition thin-layer chromatography |
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