Determination of polychlorinated biphenyls in human blood serum by SPME. |
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| Authors: | K F Poon P K Lam M H Lam |
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| Institution: | Department of Biology and Chemistry, City University of Hong Kong, Kowloon. |
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| Abstract: | We report a direct immersion SPME-GC/mu ECD method for the determination of non-volatile pollutants (Aroclor 1254) in human blood plasma using an enzymatic proteolytic approach to overcome the protein fouling problem. A nonspecific serine protease, Proteinase K, is used to remove the fouling interference. Significant improvement in relative repeatability from 28.9% to 3.4% (n = 10) was obtained. Analyte recovery up to 93% and detection limit of 1.0 ppb (total PCB) were achieved. |
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