Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability |
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Authors: | Erable Benjamin Goubet Isabelle Lamare Sylvain Legoy Marie-Dominique Maugard Thierry |
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Institution: | Laboratoire de Biotechnologies et de Chimie Bio-organique CNRS FRE 2766, Batiment Marie Curie, Université de La Rochelle, Avenue Michel Crépeau 17042 La Rochelle cedex 1, France. |
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Abstract: | Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9.0. The most effective dehalogenase activity for 1-chlorobutane conversion was detected with Rhodococcus erythropolis NCIMB13064 and Escherichia coli BL21 (DE3) (DhaA). The presence of 2-chlorobutane or propanal in the aqueous media could inhibit the 1-chlorobutane transformation. |
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