| PCR-微阵列法检测沿岸海水致病性创伤弧菌及危害评估 |
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| 引用本文： | 郑泽军,李永君,魏晓娜,朱琳,黄熙泰.PCR-微阵列法检测沿岸海水致病性创伤弧菌及危害评估[J].海洋环境科学,2009,28(2). |
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| 作者姓名： | 郑泽军 李永君 魏晓娜 朱琳 黄熙泰 |
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| 作者单位： | 1. 南开大学生命科学学院生化与分子生物学系,天津,300071
2. 南开大学环境科学与工程学院,天津,300071 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 本研究建立了PCR和寡核苷酸探针阵列杂交技术,方便、快速、准确地检测了海水中创伤弧菌的存在状况.本项检测针对创伤弧菌的两个重要毒性基因whA,viuB,其中viuB可以对普通环境株和致病株进行区分.渤海湾沿岸6个采样点,共60个沿岸海水样本被用于这一方法的评估,得到了这些采样点所在区域创伤弧菌的分布,毒性株的存在状况的信息.
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| 关 键 词： | 创伤弧菌 海水 |
Detection of Vibrio vulnificus and evaluation of virulence by PCR and microarray in coastal seawater |
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| ZHENG Ze-jun,LI Yong-jun,WEI Xiao-na,ZHU Lin,HUANG Xi-tai.Detection of Vibrio vulnificus and evaluation of virulence by PCR and microarray in coastal seawater[J].Marine Environmental Science,2009,28(2). |
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| Authors: | ZHENG Ze-jun LI Yong-jun WEI Xiao-na ZHU Lin HUANG Xi-tai |
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| Institution: | 1.Department of Biochemistry and Molecular biology;College of Life Science;Nankai University;Tianjin 300071 China;2.College of Environmental Science and Engineering;Tianjin 300071;China |
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| Abstract: | Vibrio vulnificus is a significant pathogen to human beings,and has a wide distribution in the marine environment.As the development of seawater mariculture,tourism and increasing consumption of seafood,the investigation of Vibrio vulnificus in seawater has become more and more important.In this study,a method combined use of PCR and oligonucleatide probe array hybridization was developed for the detection of Vibrio vulnificus in coastal seawater as well as investigation of virulence.The target genes used f... |
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| Keywords: | viuB whA |
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