纤维素酶、半纤维素酶降解膨化玉米秸秆工艺优化

利用纤维素酶和半纤维素酶协同降解经过膨化预处理的玉米秸秆，以提高玉米秸秆相比于单酶解的产糖量。在单因素实验的基础上，以还原糖产率为响应值，通过响应面来优化设计实验。实验数据分析得出，膨化玉米秸秆酶解的最佳工艺为：pH 4.8，液固比13：1，酶解时间60 h，酶浓度6 g/L，温度51℃。对比纤维素酶单独作用于玉米秸秆的降解效果，双酶协同酶解使酶解液的还原糖产率提高到24%，还原糖产率提高了14.3%。协同酶解的研究为木质纤维素原料的降解提供了一种新的方式。

Optimization of degradation of expanded corn straw by cellulase and hemicellulase

Cellulose and hemicellulase were used to degrade the corn straw pretreated by expanded in order to improve the sugar yield which compared with degraded only by cellulose.On basis of single factor experiment,and use reducing sugar yield as response value,optimization experiment was designed by response surface.By analysis of testing data,results showed that the best process for the expanded corn straw enzymatic hydrolysis was pH of 4.8,liquid to solid ratio of 13:1,hydrolysis time of 60 h,enzyme concentration of 6 g/L,temperature of 51℃.Compared with the results which degraded only by cellulase,the reducing sugar yield increased 14.3% and reached to 24%.The study of synergetic enzymatic hydrolysis provides a new way to degrade the lignocellulose material.