Improved method for analyzing the degradation of estrogens in water bysolid-phase extraction coupled with ultra performance liquidchromatography-ultraviolet detection |
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Authors: | Minggang Zheng Ling Wang Yuandui Bi Feng Liu |
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Affiliation: | 1. The First Institute of Oceanography, Qingdao 266101, China 2. College of Chernistry and Chemical Engineering and Environment, Qingdao University, Qingdao 266071, China |
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Abstract: | We established an improved method for the determination of four estrogens including estriol (E3), 17β-estradiol (E2), 17α-ethynyl-estrodiol (EE2) and estrone (E1) in water. The method consisted of solid-phase extraction (0.5 L water) and subsequent analysis of analytes by ultra-performance liquid chromatography (UPLC) with an ultraviolet detector (UVD). Base-line separation was achieved for all studied estrogens using a column (50 nm × 2.1 mm) packed with 1.7 μm particle size stationary phase. Recovery was higher than 88% and detection limits ranged between 12.5-23.7 ng/L for the four estrogens, with the RSD ranging from 7% to 11%. The method was successfully applied to determine E2 and EE2 in simulated natural water, which found that about 70% of E2 was degraded (with a half-life of about 30 hr) within 48 hr and about 55% of EE2 was degraded (with a half-life of about 36 hr). Low levels of El were found, however E3 was undetectable during the process. |
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Keywords: | estrogens base-lined separation half-life degradation |
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