| 甲氰菊酯降解菌JQL4-5的分离鉴定及降解特性研究 |
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| 引用本文： | 洪源范,洪青,武俊,张忠辉,李顺鹏.甲氰菊酯降解菌JQL4-5的分离鉴定及降解特性研究[J].环境科学,2006,27(10):2100-2104. |
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| 作者姓名： | 洪源范 洪青 武俊 张忠辉 李顺鹏 |
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| 作者单位： | 1. 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095
2. 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095;中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京,210008 |
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| 基金项目： | 国家自然科学基金项目(30400013,40471073);江苏省科技厅项目(BG2005322);土壤与农业可持续发展国家重点实验室开放基金项目(055112) |
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| 摘 要： | 从农药厂废水处理池的活性污泥中分离到1株能以甲氰菊酯为唯一碳源生长的细菌,命名为JQL4-5.根据其生理生化特征和16S rDNA(GenBank Accession No.DQ177525)序列相似性分析,将该菌株鉴定为鞘氨醇单胞菌属(Sphingomonas sp.).该菌株在24h内对20 mg/L的甲氰菊酯的降解率达到99.8%.降解甲氰菊酯的最适温度为30℃,pH为7.0,降解速率与初始接种量呈正相关.酶的定域试验表明,降解甲氰菊酯的酶为胞内酶.
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| 关 键 词： | 甲氰菊酯 生物降解 鞘氨醇单胞菌JQL4-5 酶的定域 |
| 文章编号： | 0250-3301（2006）10-2100-05 |
| 收稿时间： | 2005-11-08 |
| 修稿时间： | 2005-11-082005-12-29 |
Isolation, Identification and Characteristics of a Fenpropathrin-Degrading Bacterium JQL4-5 |
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| HONG Yuan-fan,HONG Qing,WU Jun,ZHANG Zhong-hui and LI Shun-peng.Isolation, Identification and Characteristics of a Fenpropathrin-Degrading Bacterium JQL4-5[J].Chinese Journal of Environmental Science,2006,27(10):2100-2104. |
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| Authors: | HONG Yuan-fan HONG Qing WU Jun ZHANG Zhong-hui and LI Shun-peng |
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| Institution: | 1. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China,1. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2. Institute of Soil Science, Chinese Academy of Science, State Key Labora,1. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China,1. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China,1. Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | A bacterium capable of utilizing fenpropathrin as sole carbon source was isolated from activated sludge collected from wastewater treating system of a pesticide manufacturer. This bacterium was identified as Sphingomonas sp. according to its physiological & biochemical analysis and the similarity analysis of its 16S rDNA sequence (GenBank Accession No. DQ177525). This bacterium could degrade 99.8% of 20 mg/L fenpropathrin in 24h. The optimal pH and temperature for the degradation were 7.0 and 30 degrees C, respectively. The degradation speed was related positively to initial inoculum size. The enzyme distribution experiment showed that the degrading-enzyme in the bacterium was endoenzyme. |
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| Keywords: | fenpropathrin biodegradation Sphingomonas sp JQL4-5 enzyme distribution |
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