Photodynamic action of some sensitizers by photooxidation of luminol |
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Authors: | Franz Wierrani Andreas Kubin Hans Loew Michael Henry Babette Spängler Klaus Bodner Werner Grünberger Robert Ebermann Gerhart Alth |
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Institution: | Viennese Society for Photophysical Diagnosis and Photodynamic Therapy, Juchgasse 25, 1030 Vienna, Austria. |
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Abstract: | We report the development of a novel simple experimental method which allows the comparison of new photosensitizers based on their production of reactive oxygen species. A high-performance liquid chromatography (HPLC) assay permits the monitoring of several substances (sensitizer, reactant and oxidized end product) simultaneously on a single chromatogram. Photoreactions were monitored throughout their course by the HPLC assay surveying the sensitizers' efficiency of singlet oxygen production by the oxidative decomposition of luminol. Several photosensitizers were tested: Rose Bengal, Methylene Blue, Protoporphyrin IX, Photosan III, Photofrin, Hypericin and Pseudohypericin. Additionally, photoreactions were monitored by a standard pO2 detection system. The measurements of the two detection methods were strongly correlated. Rose Bengal proved to be the most efficient photosensitizer, clearly decreasing the luminol concentration and causing a corresponding increase in aminophthalic acid. Our experiments show that when factors necessary for photochemical reactions are absent or are blocked (antioxidants), no reaction can be detected. |
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