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辽河流域水诱发蚕豆根尖细胞遗传损伤的研究
引用本文:张利红,徐成斌,陈忠林,苏丹,王家懿.辽河流域水诱发蚕豆根尖细胞遗传损伤的研究[J].环境科学与技术,2009,32(12).
作者姓名:张利红  徐成斌  陈忠林  苏丹  王家懿
作者单位:1. 辽宁大学环境学院,辽宁,沈阳,110036
2. 辽宁省环境科学研究院,辽宁,沈阳,110031
3. 冻北育才学校生命科学实验室,辽宁,沈阳,110179
基金项目:国家水体污染控制与治理科技重大专项 
摘    要:以辽河流域12个不同断面的河水为诱变剂,运用蚕豆根尖微核检测技术和染色体畸变实验方法,测定蚕豆根尖细胞的有丝分裂指数、微核率和染色体畸变率。结果表明:不同断面的河水均能降低蚕豆根尖细胞有丝分裂指数,能诱发较高频率的微核和染色体畸变,产生染色体断片、核突起和核碎裂。所有样点微核率和染色体畸变率均高于对照组。根据采样点水质污染指数分析可知,福德店水质属重度污染,东辽河、条子河、红庙子水质属中度污染,招苏台河水质属轻度污染。

关 键 词:辽河  蚕豆  微核  染色体畸变

Genetic Damage of Root Tip Cells in Broad Bean Plant (Vicia faba) Induced by Water in Liao River Valley
ZHANG Li-hong,XU Cheng-bin,CHEN Zhong-lin,SU Dan,WANG Jia-yi.Genetic Damage of Root Tip Cells in Broad Bean Plant (Vicia faba) Induced by Water in Liao River Valley[J].Environmental Science and Technology,2009,32(12).
Authors:ZHANG Li-hong  XU Cheng-bin  CHEN Zhong-lin  SU Dan  WANG Jia-yi
Institution:ZHANG Li-hong1,XU Cheng-bin1,CHEN Zhong-lin1,SU Dan2,WANG Jia-yi3 (1.School of Environmental Science,Liaoning University,Shenyang 110036,China,2.Liaoning Academy of Environmental Science,Shenyang 110031,3.Life Science Laboratory,Northeastern Yucai School,Shenyang110179,China)
Abstract:Water in 12 sections of Liao River Valley was sampled as mutagen and micronucleus test and chromosome aberration assay were used to detect mitotic index,micronucleus ratio and chromosome aberration ratio of root tip cells in broad bean plant(Vicia faba). Results showed that water in different sections can decrease the mitotic index in Vicia faba root tip cells,induce higher ratios of either micronucleus or chromosome aberration and produce chromosomal segments,nuclear protuberances and fragments. Micronucle...
Keywords:Liao River Valley  broad bean plant(Viciafaba)  micronucleus  chromosome aberration
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