五氯酚对生物膜活细胞含量及分布的影响研究

以废水处理生物膜为研究对象,建立了表征生物膜细胞活性的磷脂测定方法.研究中采用冷冻切片技术,以150μm为一层,考察生物膜不同深度的磷脂浓度.结果表明,当五氯酚浓度为零时,生物膜磷脂含量随深度方向呈近似的正态分布,磷脂含量的绝对值在(1246.2±217.4)～(527.2±95.3)μg/L之间(n=4),峰值出现在...

Effect of PCP on the Living Cell Content and Distribution in Biofilms

The phospholipids method for the determination of the living cell content in the biofilm was established to study the wastewater biofilm. With the micro-slicing technology, the biofilm was divided into 150 microm-layer for the phospholipids analysis. Results showed the phospholipids were approximately normal distribution along the biofilm depth without no addition of pentachlorophenol (PCP). The absolute values were between (1 246.2 +/- 217.4) microg/L and (527.2 +/- 95.3) microg/L with the maximum occurring at the depth of (0.60 +/- 0.15) microm, indicating the highest concentrations of the living cell in the biofilm. With the PCP concentrations of 5 microg/L, 10 microg/L and 20 microg/L and the exposure times of 2 h, 6 h and 24 h, respectively, the results of the orthogonal experiments showed the value of phospholipids decreased greatly along the biofilm depth only with the exception of 5 microg/L PCP concentration and 2 h exposure time, with a concentration decline by up to 70% especially close to the peak of phospholipids distribution. Results proved that PCP has an adversely influence on the living cells, and the influence can be enhanced with the increase of PCP amount and the exposure time.