| 生物表面活性剂对铜绿假单胞菌摄取烷烃的强化机制 |
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| 引用本文： | 梁生康,苏荣国,王修林,周爱华,汪卫东.生物表面活性剂对铜绿假单胞菌摄取烷烃的强化机制[J].应用与环境生物学报,2006,12(4):566-569. |
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| 作者姓名： | 梁生康 苏荣国 王修林 周爱华 汪卫东 |
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| 作者单位： | 1. 中国海洋大学化学化工学院,山东青岛,266003
2. 中国石化胜利油田有限公司采油工艺研究院,山东东营,257000 |
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| 基金项目： | 国家科技攻关项目;山东省青岛市科技发展基金 |
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| 摘 要： | 考察了2株铜绿假单胞菌(Pseudomonasaeruginosa)以正十六烷为底物生长的不同方式,并初步探讨了生物表面活性剂在烃类降解菌摄取烷烃过程中的作用机制.菌株O-2-2在正十六烷中的生长明显快于PS-1,生长过程中O-2-2分泌出鼠李糖脂生物表面活性剂,正十六烷被完全乳化.另外,O-2-2菌细胞表面疏水性高于PS-1,而加入鼠李糖脂使得菌体细胞中脂多糖含量减少,菌细胞表面疏水性明显提高.上述结果表明,鼠李糖脂主要通过乳化疏水性底物和提高降解菌表面疏水性两种机制强化铜绿假单胞菌(P.aeruginosa)对烷烃的摄取.图6表1参14
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| 关 键 词： | 铜绿假单胞菌 正十六烷 生物表面活性剂 鼠李糖脂 疏水性 |
| 收稿时间： | 2005-01-11 |
| 修稿时间： | 2005-05-18 |
Biosurfactant-enhanced Mechanisms During Uptake of Alkane by Pseudomonas aeruginosa |
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| LIANG Shengkang,SU Rongguo,WANG Xiulin,ZHOU Aihua,WANG Weidong.Biosurfactant-enhanced Mechanisms During Uptake of Alkane by Pseudomonas aeruginosa[J].Chinese Journal of Applied and Environmental Biology,2006,12(4):566-569. |
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| Authors: | LIANG Shengkang SU Rongguo WANG Xiulin ZHOU Aihua WANG Weidong |
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| Institution: | College of Chemistry and Chemical Engineering, Ocean University of China ,Qingdao 266003, Shandong, China;1 Oil Production Research lnistitute of SINOPEC Shengli Oilfield Limited Company, Dongying 257000, Shandong, China |
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| Abstract: | Two strains of Pseudomonas aeruginosa grown on n-hexadecane and the roles of biosurfactant involved in alkane assimilation by hydrocarbon-degrading bacteria were investigated. P. aeruginosa O-2-2 grew on n-hexadecane much faster than P. aeruginosa PS-1. During the growth on n-hexadecane, O-2-2 produced an extracellular rhamnolipid biosurfactant and n-hexadecane was emulsified completely. In addition, O-2-2 exhibited higher cell surface hydrophobicity than PS-1. The presence of rhamnolipid biosurfactant caused a significant reduction of lipopolysaccharide in cell outer membrane, resulting in cell surface hydrophobicity increase. These findings indicated that emulsification of hydrophobic substrate and enhancement of cell surface hydrophobicity were two possible mechanisms, by which rhamnolipid could enhance alkane assimilation. Fig 6, Tab 1, Ref 14 |
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| Keywords: | Pseudomonas aeruginosa n-hexadecane biosurfactant rhamnolipid hydrophobicity |
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