原噬菌体诱导法在环境诱变剂研究中的应用

应用原噬菌体诱导法检测35种不同化学物质的潜在遗传毒性,S_9作代谢激活系统。试验结果有13个样品具有诱变性(占37.1％),其中有10个样品在S_9激活下和3个样品无论有与无S_9活化均能诱导溶源性大肠杆菌释放λ噬菌体。检出的阳性样品中有些为已知的诱变剂和致癌剂(黄曲霉毒素B_1)或抗癌药(丝裂霉素C)。提示诱导检测法作为环境遗传毒物的预警系统初筛潜在致癌危险物质是一种极为有用的手段。

Application of Inductest in Research of Environmental Mutagens

The possible mutagenic activity of 35 different chemicals has been tested with inductest, in which S₉ mixture was used as a metabolizing system. The results showed that 13(37%)chemicals had mutagenic activity, 10 of these chemicals gave positive reaction in inductest in the presence of S₉ mixture, three chemicals gave positive reaction in inductest in the presence of S₉ mixture or without it. Some of the chemicals are known as potent mutagens and carcinogens(aflatoxin B₁)or anticancer drugs(mitomycin C). It is considered that inductest is an ef-fective method in research of environmental mutagens.