| 一株降解微囊藻毒素菌种的鉴定及其活性研究 |
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| 引用本文： | 吴涓,钟升,王光云,李玉成.一株降解微囊藻毒素菌种的鉴定及其活性研究[J].中国环境科学,2011,31(1):116-122. |
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| 作者姓名： | 吴涓 钟升 王光云 李玉成 |
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| 作者单位： | 安徽大学生命科学学院,安徽,合肥,230039 |
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| 基金项目： | 安徽省自然科学基金，中石化石油勘探开发研究院项目 |
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| 摘 要： | 从巢湖水体中分离出一株能够降解微囊藻毒素(MCs)的菌株M9,通过生理生化实验以及16S rDNA序列比对和序列分析,表明该菌株16S rDNA的全序列与吉氏库特菌kurthia gibsonii的相似性达99%.研究了pH值、外加碳源、氮源以及金属离子对菌株M9降解MCs活性的效应,发现菌株M9降解MCs的最适pH值为7.0.乙醇可显著提高MCs的降解活性,在48h内对微囊藻毒素RR(MC-RR)和微囊藻毒素LR(MC-LR)的降解率分别达到70.0%和81.4%.以硫酸铵为氮源时该菌株对MC-RR和MC-LR的降解率最高,分别为70.4%和80.9%.金属离子Cu2+、Zn2+和Mn2+对菌株M9降解MCs有明显的促进作用,48h时MC-RR和MC-LR的降解率分别达到了85%和93%以上;而Fe3+和Mg2+对降解过程的促进作用不如Cu2+、Zn2+和Mn2+.
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| 关 键 词： | 微囊藻毒素 鉴定 16S rDNA 生物降解 活性 |
| 收稿时间： | 2010-05-14; |
Identification and activity of a bacterial strain for the biodegradation of microcystins |
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| WU Juan,ZHONG Sheng,WANG Guang-yun,LI Yu-cheng.Identification and activity of a bacterial strain for the biodegradation of microcystins[J].China Environmental Science,2011,31(1):116-122. |
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| Authors: | WU Juan ZHONG Sheng WANG Guang-yun LI Yu-cheng |
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| Institution: | WU Juan,ZHONG Sheng,WANG Guang-yun,LI Yu-cheng(School of Life Science,Anhui University,Hefei 230039,China). |
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| Abstract: | A microcystin-degrading bacterial strain M9 isolated from Chaohu Lake was identified by physiological-biochemical characteristics and homology analysis of 16S rDNA gene sequence.BLAST sequence analysis showed that it had a 99% similarity to kurthia gibsonii.The effects of pH,additional carbon and nitrogen sources,and metal ions on the biodegradation activity of microcystins(MCs) by strain M9 were investigated.pH7.0 was the best value for the biodegradation of MCs by strain M9.Compared with glucose and glyce... |
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| Keywords: | 16S rDNA |
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