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| 广东某农村塘坝饮用水污染的微生物溯源 | |
| 引用本文： | 冯广达,邓名荣,郭俊,朱红惠,羊宋贞,梁浩亮,朱昌雄.广东某农村塘坝饮用水污染的微生物溯源[J].中国环境科学,2011,31(1):96-104. |
| 作者姓名： | 冯广达 邓名荣 郭俊 朱红惠 羊宋贞 梁浩亮 朱昌雄 |
| 作者单位： | 1. 广东省微生物研究所广东省华南应用微生物重点实验室,广东,广州,510070 2. 惠州市水产科学技术研究所,广东,惠州,516007 3. 中国农业科学院农业环境与可持续发展研究所,北京,100081 |
| 基金项目： | "十一五"国家水体污染控制与治理科技重大专项 |
| 摘 要： | 以大肠杆菌为指示微生物,采用脉冲场凝胶电泳(PFGE)、肠杆菌间重复一致序列PCR(ERIC-PCR)和BOX插入因子PCR(BOX-PCR)3种分子分型方法对广东省某典型农村塘坝饮用水污染来源进行了追踪研究,利用软件Quantity One进行分析.结果表明,浅层井水与其近处的池塘水存在大肠杆菌的克隆现象,两者可能存在着共同的污染传播载体,或是浅层井水受到了池塘水的污染.3种方法的分型能力从强到弱依次为PFGE、BOX-PCR、ERIC-PCR.其中, BOX-PCR最宜在水体污染的微生物溯源中应用.鉴于分离培养的溯源方法存在的缺陷,应加强与非培养微生物溯源相结合进行分析以使结果更加准确. |
| 关 键 词： | 微生物溯源 大肠杆菌 PFGE ERIC-PCR BOX-PCR |
| 收稿时间： | 2010-05-07; |
Microbial source tracking of the pollution in pond-drinking water in a country of Guangdong |
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| FENG Guang-da,DENG Ming-rong,GUO Jun,ZHU Hong-hui,YANG Song-zhen,LIANG Hao-liang,ZHU Chang-xiong.Microbial source tracking of the pollution in pond-drinking water in a country of Guangdong[J].China Environmental Science,2011,31(1):96-104. | |
| Authors: | FENG Guang-da DENG Ming-rong GUO Jun ZHU Hong-hui YANG Song-zhen LIANG Hao-liang ZHU Chang-xiong |
| Institution: | FENG Guang-da1,DENG Ming-rong1,GUO Jun1,ZHU Hong-hui1,YANG Song-zhen1,LIANG Hao-liang2,ZHU Chang-xiong3(1.Guangdong South China Key Laboratory of Microbial Application,Guangdong Institute of Microbiology,Guangzhou 510070,China,2.Huizhou Fishery Institute of Science and Technology,Huizhou 516007,3.Institute of Agricultural Environment and Sustainable Development,Chinese Academy of Agricultural Sciences,Beijing 100081,China). |
| Abstract: | Pollution sources of the typical rural pond-drinking water were studied in a country of Guangdong using molecular typing methods such as pulsed-field gel electrophoresis(PFGE),ERIC-PCR and BOX-PCR.Escherichia coli was the indicating microorganism in microbial source tracking(MST).The fingerprints of E.coli from different samples were analyzed using Quantity One.It was showed that the superficial layer well had the same genotyping E.coli with the pond water beside it.Therefore,they might have the same pollut... |
| Keywords: | PFGE ERIC-PCR BOX-PCR |
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