微生物苯丙氨酸解氨酶(PAL)/肉桂酸途径生物转化制L-苯丙氨酸酶源菌种选育

Ｌ苯丙氨酸（Ｌｐｈｅｎｙｌａｌａｎｉｎｅ，ＬＰｈｅ）是生化营养的必需氨基酸之一，作为临床医药用氨基酸输液、食品、饲料添加剂的必需成份和一些抗癌药物苯丙氨酸氮芥、甲酰溶肉瘤素等合成的中间体而得到广泛应用．自１９６５年天冬甜肽（Ａｓｐａｒｔａｍｅ，ＡＰＭ，阿斯巴甜）发现并开发生产以来，这一新型甜味剂日渐风行，导致对高光学纯度ＬＰｈｅ这一主要构成原料需求量激增，人们纷纷开展ＬＰｈｅ的研制生产．在众多生物合成途径中，肉桂酸酶法转化生产ＬＰｈｅ的研究开发因其原料易得价廉，倍受国内外的许多公司和…

BREEDING OF MICROBIAL STRAINS CONTAINING PHENYLALANINE AMMONIA LYASE (PAL) FOR THE BIOCONVERSION OF TRANS CINNAMIC ACID INTO LPHENYLALANINE

Phenylalanine ammonia lyase (PAL) has great values in industrial and potential medical applications, especially in the production of L phenylalanine from trans cinnamic acid. Strain breeding is important for the development and applications of this bioprocess. In this article, mutiple ways for breeding of microbial strains containing PAL were reviewed in details, including direction screening, enrichment culture technique for isolating strains from nature sources, phsical and chemical mutagenesis for strain improvement, PAL molecular clone and genetic engineering, etc. The significance of protoplast techniques for breeding of Rhodotorula strains containing PAL was analysed and discussed.