癸二烯醛对3种常见浮游植物光合色素的影响

本研究选取东海原甲藻（Prorocentrum donghaiense）、并基角毛藻（Chaetoceros decipiens）和小普林藻（Prymnesium parvum）等3种常见海洋浮游藻类为材料，将处于指数生长期的藻细胞置于不同浓度的癸二烯醛（2E，4E-Decadienal，DEC）中，癸二烯醛是一种毒性较强的常见多不饱和醛，观察癸二烯醛对藻类光合色素种类及含量的影响。利用高效液相色谱技术检测到东海原甲藻含有5种光合色素，并基角毛藻含有8种光合色素，小普林藻含有9种光合色素，3种藻类的共有色素有4种。经浓度为1 mg/L癸二烯醛处理后，3种藻类的光合色素浓度无显著变化。癸二烯醛浓度为3 mg/L时，对东海原甲藻光合色素浓度产生显著的抑制作用，其所有色素浓度在第一天内几乎都未检测到；而并基角毛藻的硅甲藻黄素和硅藻黄素浓度均在第一天显著增加，其他色素浓度则无显著变化；小普林藻的硅甲藻黄素、硅藻黄素、岩藻黄素的浓度在实验的不同时间有不同程度下降，其余色素不受该浓度的癸二烯醛影响。当癸二烯醛浓度达到5 mg/L时，并基角毛藻中除玉米黄素外的其他7种光合色素浓度显著下降；小普林藻中除玉米黄素外的多种色素浓度受到显著影响。

Effects of 2E, 4E-decadienal on the photosynthetic pigments of three species of phytoplankton

Prorocentrum donghaiense, Chaetoceros decipiens and Prymnesium parvum were exposed to Decadienal (2E, 4E-Decadienal, DEC) which was a common toxic polyunsaturated aldehydes at different concentrations, the species and contents of photosynthetic pigments were monitored frequently.The HPLC results showed that 5, 8, 9 types of photosynthetic pigments were detected in P.donghaiense, C.decipiens, P.parvum respectively, in which there were four identical pigments.There was no significant difference in the concentrations of photosynthetic pigments of three species of algae with DEC at 1 mg/L.However, DEC at 3 mg/L inhibited P.donghaiense and all of its pigment concentrations almost not detected within the first day; The concentration of diadinoxanthin and diatoxanthin of C.decipiens increase significantly on the first day while the other pigments were not significant changed.The concentration of diadinoxanthin, diatoxanthin and fucoxanthin of P.parvum decreased to varying degrees with time while the remaining pigments were not affected by DEC at 3 mg/L.Except for zeaxanthin, concentrations of the other seven types of photosynthetic pigments in C.decipiens decreased significantly by DEC at 5 mg/L, A similar trend of the pigments concentration changes were also observed in P.parvum at the same DEC concentrations, in which except for zeaxanthin, seven types of pigments out of nine were significantly affected.