The fractionation of extracts of ambient particulate matter for mutagenicity testing |
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| Affiliation: | 1. Department of Biotechnology, Thapar Institute of Engineering and Technology (TIET), Patiala 147001, India;2. Department of Biotechnology, Dr B R Ambedkar National Institute of Technology Jalandhar, G.T. Road Bypass, Jalandhar 144027, India;3. College of Biosystems Engineering and Food Science, Zhejiang University, China;4. Gujarat Pollution Control Board, Gandhinagar 382 010, Gujarat, India;1. Laboratory of Technology and Pharmaceutical Biotechnology (Tecbiofar), College of Pharmacy, Federal University of Rio Grande do Norte, Street General Gustavo Cordeiro de Farias, S/N, Petrópolis, Natal, 59012-570, Brazil;2. Graduate Program of Chemistry, Chemistry Institute, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil;3. Laboratory of Biotechnology of Natural Biopolymers, Department of Biochemistry, Bioscience Center, Federal University of Rio Grande do Norte, Avenue Senador Salgado Filho, 3000, Lagoa Nova, 59072-970, Natal, Brazil;4. Laboratory of Phytochemical Bioprospecting, Department of Chemistry, Campus Dois Irmãos, Federal Rural University of Pernambuco, Street Manuel de Medeiros, s/n, Dois Irmãos, 52171-900, Recife, Brazil |
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| Abstract: | Extracts of air paniculate matter were fractionated by column chromatography over three different adsorbentia. It is shown that chromatography over alumina and over silicagel should be avoided, because of the low recovery of mutagenicity involved. On the other hand, fractionation over XAD-2 is strongly recommended, because of its ease of use and the superior recoveries of both mass and mutagenicity. HPLC subfractionation of the most mutagenic XAD-2 fraction showed that the mutagenicity cannot be attributed to a limited number of compounds. |
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