| 薄层色谱紫外分光光度法定量测定微生物发酵产物辅酶Q10 |
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| 引用本文： | 郜晓峰,范成英,何开泽,官家发.薄层色谱紫外分光光度法定量测定微生物发酵产物辅酶Q10[J].应用与环境生物学报,2006,12(3):420-423. |
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| 作者姓名： | 郜晓峰 范成英 何开泽 官家发 |
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| 作者单位： | 1. 中国科学院成都生物研究所,成都,610041;中国科学院研究生院,北京,100049
2. 中国科学院成都生物研究所,成都,610041 |
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| 摘 要： | 报道了一种微生物发酵液中辅酶Q10的定量检测方法.该方法分为两个步骤:第一步是离心收集菌体和结合使用超声波处理的辅酶Q10丙酮抽提;第二步是硅胶薄层色谱,根据标样位置取产物斑点乙醇洗脱后用紫外分光光度法测定辅酶Q10含量.结果表明,该方法具有操作简便、重复性好、相对误差小等优点,可用于细菌发酵生产辅酶Q10研究过程中发酵产物的快速定量检测.图2表2参6
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| 关 键 词： | 辅酶Q10 薄层色谱 紫外定量分析 |
| 收稿时间： | 2005-08-22 |
| 修稿时间： | 2005-11-01 |
Quantitative Analysis of Coenzyme-Q10 from Microbial Culture by TLC-UV Spectrophotometry |
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| GAO Xiaofeng,FAN Chengying,HE Kaize,GUAN Jiafa.Quantitative Analysis of Coenzyme-Q10 from Microbial Culture by TLC-UV Spectrophotometry[J].Chinese Journal of Applied and Environmental Biology,2006,12(3):420-423. |
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| Authors: | GAO Xiaofeng FAN Chengying HE Kaize GUAN Jiafa |
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| Institution: | 1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | A quantitative analysis of coenzyme-Q_(10) from microbial culture was developed.Using acetone sonic treatment,CoQ_(10) was extracted from microbial cell and TLC-UV spectrophotometry method was then used for quantitative analysis of CoQ_(10).The method was found with good repeatability and stability.With this method,the CoQ_(10) in microbial culture could be easily and rapidly analysed.Fig 2,Tab 2,Ref 6 |
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| Keywords: | coenzyme-Q_(10) TLC-spectrophotometry UV-quantitative analysis |
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