Detection of hormone mimics in water using a miniturised SPR sensor |
| |
Authors: | Sesay A M Cullen D C |
| |
Institution: | (1) Cranfield Biotechnology Centre, Institute of BioScience and Technology, Cranfield University at Silsoe, Silsoe, Bedfordshire, U.K.;(2) Cranfield Biotechnology Centre, Institute of BioScience and Technology, Cranfield, University at Silsoe, Silsoe, Bedfordshire, U.K. |
| |
Abstract: | The ubiquitous presence of chemicals, both natural andsynthetic, in the environment with the potential to mimichormones that may in turn interfere with the endocrinesystem in both wildlife and humans has in the last decadebecome a major international concern. Hormone mimics orendocrine disrupting compounds (EDCs) are especiallyprevalent in surface and waste-waters and therefore, thereis a need for an at-source or at-line analytical device forthe monitoring of EDC levels.We have incorporated a miniature integrated surface plasmonresonance (SPR) liquid sensor from Texas Instruments into afield analyser and developed a competition/inhibition assayfor a model estrogenic compound in aqueous samples. Theanalyser has the potential for in situ and semi-continuous analysis of EDCs. A novel regeneration schemeemploying the use of a domestic laundry detergent has beenused to remove immobilised assay components between eachassay cycle. The resultant re-usable sensor has beendemonstrated using estrone-3-glucuronide (E3G) as a modelEDC and an anti-E3G antibody producing a current detectionrange of 10 to 150 ng mL-1. |
| |
Keywords: | endocrine disrupting compounds estrone-3-glucuronide hormone mimics miniturised SPR sensor surface plasmonresonance |
本文献已被 PubMed SpringerLink 等数据库收录! |
|