功能微生物对污染农田土壤中铅锌的溶出实验

用微生物修复重金属污染的土壤是当今的研究热点之一。试图寻找一类可以增加土壤中重金属铅、锌的可溶态的功能微生物,并研究该微生物在不同土壤含水率和不同投菌比条件下对铅、锌溶出作用的影响。最终筛选出符合条件的菌JK3,并发现当土壤含水率为35%,投菌比为0.15 mL/g土(菌液OD=1)时,土壤中铅和锌的可溶态增加量最多。其中,可溶态铅的含量由原来的0.49 mg/kg增加到了5.04 mg/kg,可溶态锌的含量由原来的2.23 mg/kg增加到了22.44mg/kg。该方法为后续用植物提取或化学淋洗等方法将土壤中的重金属彻底去除提供了可能。

Experiment on dissolution of Pd and Zn in heavy metals contaminated agricultural soil by functional microorganisms

Remediation of heavy metals contaminated soil by microbial technology has attracted much more attention from the researchers recently. Experiments were carried out to investigate the capabilities of several kinds of microorganisms to transfer the insoluble heavy metal species (including Pb and Zn) into the soluble ones, and the effects of soil moisture and the ratio of microorganism solution volume to soil weight (M/S) were investigated. The microorganism JK3 is found to be the optimal strain to increase concentrations of soluble Pb and Zn in soil, and concentrations of soluble Pb and Zn reach the maximum with the soil moisture content of 35% and M/S of 0.15 mL/g (OD=1). The concentration of soluble Pb increased from 0.49 mg/kg to 5.04 mg/kg while that of Zn from 2.23 mg/kg to 22.44 mg/kg. This microbial remediation technology provides the possibility to remove heavy metals from soil thoroughly for the subsequent phyto-extraction by hyperaccumulators or chemical washing process.