Response of the Ascorbate-Peroxidase of Selenastrum capricornutum to Copper and Lead in Stormwaters |
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| Authors: | M-Y Wong K R Sauser K-T Chung T-Y Wong J-K Liu |
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| Institution: | (1) Water Quality Control Plant, City of Palo Alto, CA, U.S.A.;(2) Department of Microbiology and Molecular Cell Sciences, The University of Memphis, Memphis, TN, U.S.A.;(3) Department of Microbiology and Molecular Cell Sciences, The University of Memphis, Memphis, Tennessee, 38152, U.S.A.;(4) Department of Biological Sciences, National Sun Yat-sen University, Taiwan, Republic of China |
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| Abstract: | The green alga Selenastrum capricornutum expresses a uniqueascorbate peroxidase, that responds to copper and lead. Attemptswere made to test if this peroxidase could be used to monitor thelevels of copper and lead in natural waters. When S.capricornutum was exposed to a stormwater sample, the specificactivity of the peroxidase in the cell extract was commensuratewith the combined copper and lead contents in the sample. Theperoxidase responses were also correlated with the 96 hr biomasstoxicity assay of S. capricornutum. However, unlike thebiomass toxicity assay, the peroxidase activity was not affectedby the anions in the samples. The use of this peroxidase can beused as a marker for testing heavy metal toxicity in the water. |
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| Keywords: | bioindicator metal toxicity peroxidase Selenastrum capricornutum 96 hr algal toxicity assay" target="_blank">gif" alt="sim" align="MIDDLE" BORDER="0">hr algal toxicity assay |
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