| 利用GFP和抗性双类型标记监测联合固氮菌在玉米根际的定殖 |
| |
| 引用本文： | 董越梅,安千里,李久蒂,朱至清. 利用GFP和抗性双类型标记监测联合固氮菌在玉米根际的定殖[J]. 应用与环境生物学报, 2000, 6(1): 61-65 |
| |
| 作者姓名： | 董越梅 安千里 李久蒂 朱至清 |
| |
| 作者单位： | 中国科学院植物研究所,北京,100093 |
| |
| 基金项目： | 国家高科技研究发展计划 86 3项目资助 |
| |
| 摘 要： | 将来自质粒ｐＫＲＰ１０、ｐＫＲＰ１１和ｐＫＲＰ１２的氯霉素、卡那霉素和四环素抗性基因分别插入质粒ＧＦＰｍｕｔ２中ｇｆｐ基因下游的ＰｓｔＩ位点，得到ｇｆｐ和不同抗性基因共存的重组质粒，转化Ｅｎｔｅｒｏｂａｃｔｅｒ ｇｅｒｇｏｖｉａｅ ５７－７野生型菌株和耐铵工程菌Ｅ７后，得到既有抗生素抗性又在蓝光下呈现亮绿荧光的菌株。用它们接种玉米后，利用这两种选择标记双重筛选重新分离到的细菌确定了接种菌在玉米幼苗
|
| 关 键 词： | 联合固氮菌 工程菌监测 GFP 抗性 双标记基因 |
| 修稿时间： | 1999-03-04 |
USE OF GFP AND ANTIBIOTIC RESISTANCE AS SELECTIVE MARKERS TO MONITOR COLONIZATION OF ASSOCIATED NITROGEN-FIXING BACTERIA IN MAIZE RHIZOSPHERE |
| |
| DONG Yuemei,AN Qianli,LI Jiudi,ZHU Zhiqing. USE OF GFP AND ANTIBIOTIC RESISTANCE AS SELECTIVE MARKERS TO MONITOR COLONIZATION OF ASSOCIATED NITROGEN-FIXING BACTERIA IN MAIZE RHIZOSPHERE[J]. Chinese Journal of Applied and Environmental Biology, 2000, 6(1): 61-65 |
| |
| Authors: | DONG Yuemei AN Qianli LI Jiudi ZHU Zhiqing |
| |
| Abstract: | Thechloramphenicol gene of plasmid pKRP10, the kanamycin gene of plasmid pKRP11 andtetracycline gene of plasmid pKRP12 were cloned into the PstI site on the downstream ofgfp gene of plasmid GFPmut2 respectively, the recombined plasmids had the gfp anddifferent antibiotic resistance genes . These plasmids were transformed into Enterobactergergoviae 57-7 and genetically engineered strain E7 respectively, the resultingtransformants exhibited antibiotic resistance and appeared bright green under blue light.Based on the two kinds of selective markers above, the numbers of these transformantscolonizing on maize roots were confirmed. The colonization of gfp labeled bacteria on themaize roots as well as their distribution in the soil was observed by fluorescentmicroscopy. Plate 1, Fig 1, Tab 2, Ref 15 |
| |
| Keywords: | gfp antibiotic resistance genes colonization |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
