Formation of singlet oxygen during farmorubicin oxidation. |
| |
Authors: | I Kruk T Michalska A K?adna |
| |
Institution: | Institute of Physics, Technical University of Szczecin, Poland. kruk@arcadia.tuniv.szczecin.pl |
| |
Abstract: | The enhanced generation of singlet oxygen (1O2) during oxidation of farmorubicin in the Co(II) + H2O2 system was studied using chemiluminescent, fluorescent and spectrophotometic techniques. The influence of 1O2-quenchers, catalase, superoxide anion radical (O2*-) and hydroxyl radical (HO*) scavengers on the light emission was studied. The spectrophotometric determination of 1O2 was based on bleaching of p-nitrosodimethylaniline caused by an intermediate product of the reaction of 1O2 with imidazole, and was followed by monitoring the decrease in optical density at 440 nm. |
| |
Keywords: | |
|
|