微囊藻毒素-LR对罗非鱼鳃组织活性氧自由基含量及相关抗氧化酶活性的影响

应用腹腔注射的方式,研究了微囊藻毒素MC-LR对罗非鱼（Oreochromis niloticus）鳃组织活性氧自由基含量（ROS）及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性的影响。结果表明：在MC-LR的胁迫下,罗非鱼鳃组织产生了大量的ROS,并引起了SOD和CAT 2种抗氧化酶活性的变化。SOD和CAT在清除过量的ROS时表现出的动态变化过程不同,SOD的变化曲线是多阶段的,而CAT则表现为先诱导后恢复的趋势。SOD和CAT在变化趋势上有所不同,但均表现为明显的时间与效应的正相关关系和剂量与效应的正相关关系。这也为将鳃组织中SOD和CAT作为抗氧化生物标志物来监测微囊藻毒素对罗非鱼胁迫作用提供了一定的参考。试验为研究微囊藻毒素对罗非鱼的生态毒理学作用提供相应的资料,并为罗非鱼质量安全问题的研究打下一定的基础。

The effect of MC-LR on reactive oxygen species and its related enzymes activity in gill of tilapia(Oreochromis niloticus)

This experiment focused on the effect of microcystin-LR on reactive oxygen species and its related enzymes activity（superoxide dismutase,SOD and catalase,CAT） in gill of tilapia（Oreochromis niloticus）.The results showed that surplus ROS,inducing the variation of antioxidant enzymes SOD and CAT was generated in gill by the stress of microcystin-LR.SOD and CAT had different dynamic processes,especially,the activity of SOD had multistage curves,but the activity of CAT was inhibited firstly,and induced afterwards.Although the trends of SOD and CAT were different,time-depend and dose-depend effects had been displayed evidently.These results showed that they were maybe biomarkers to monitor the toxicology of mycrocystin-LR to tilapia fish.By the experiment,more details would be provided to study the ecotoxicology and quality safety of microcystin-LR to tilapia.