锰矿石人工湿地中去除双氯芬酸的机理研究

以双氯芬酸为目标有机药物污染物，探究其在以锰矿石为填料并接种了异化金属还原菌（Geobactermetallireducens，简称GS-15细菌）的垂直流人工湿地中的去除过程.结果表明，双氯芬酸在锰矿石人工湿地中的平均去除率最高为23.56%.采用XRD和XRF对反应前后的锰矿石进行物相和元素相对含量分析，得出锰（Mn）是参与降解双氯芬酸的关键响应元素.GS-15细菌利用锰矿石发生异化还原对双氯芬酸的氧化降解是锰矿石人工湿地对双氯芬酸的主要去除途径.此外，利用液质联用技术（LC-MS/MS）对降解产物进行鉴定，发现双氯芬酸被降解生成了5-羟基双氯芬酸、双氯芬酸-2，5-亚氨基琨和1，3-二氯苯三种降解产物，该研究成果对有机药物废水的深度处理提供新的方法和理论指导.

Mechanisms of diclofenac removal in manganese ore constructed wetland

This study investigated diclofenac (DFC) removal in a vertical flow constructed wetland, in which manganese ores were used as substrates, and dissimilatory metal reducing bacteria (Geobactermetallireducens, GS-15) were added as inoculum. The results indicated that maximum average removal of DFC was 23.56% in this wetland. After analyzing the manganese ores by XRD and XRF before and after the experiments, Mn was identified as the key element participating DFC removal. The main DFC removal pathway in the wetland is the dissimilatory Mn(IV) reduction. In this process, DFC was degraded by GS-15using manganese ores as the electron acceptor. In addition, LC-MS/MS analysis was used to identify the degradation intermediates of DCF. Three intermediates are identified, namely 5-hydroxydiclofenac (5-OH-DCF), diclofenac-2, 5-iminoquinone (DCF-2, 5-IQ), and 1,3-dichlorobenzene (1,3-DCB). The results of this research provide new insight and theoretical guidance on the advanced technologies for pharmaceutical treatment in wastewater.