| 芦苇抑藻化感物质的分离及其抑制蛋白核小球藻效果研究 |
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| 引用本文： | 李锋民,胡洪营.芦苇抑藻化感物质的分离及其抑制蛋白核小球藻效果研究[J].环境科学,2004,25(5):89-92. |
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| 作者姓名： | 李锋民 胡洪营 |
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| 作者单位： | 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京,100084 |
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| 基金项目： | 国家重点基础研究发展规划(973)项目(G199045711) |
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| 摘 要： | 采用乙醇提取、溶剂萃取法从芦苇中分离出2组能抑制蛋白核小球藻生长的化感物质. 该2组化感物质对蛋白核小球藻的半效应浓度(EC50)分别为0.68mg/L和2.65mg/L. 将前者用碱性氧化铝层析柱分离得到一组抑藻活性更强的化感物质和一组能够促进蛋白核小球藻生长的化感物质: 前者对蛋白核小球藻的EC50为0.50mg/L, 是目前报道的抑藻能力最强的化感物质; 后者在浓度为0.63mg/L时能显著促进蛋白核小球藻的生长. 芦苇化感物质可以用来控制淡水中有害藻类.
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| 关 键 词： | 芦苇 蛋白核小球藻 抑制 化感作用 |
| 文章编号： | 0250-3301(2004)05-0089-04 |
| 收稿时间： | 2003/12/1 0:00:00 |
| 修稿时间： | 2004/3/24 0:00:00 |
Isolation and Effects on Green Alga Chlorella pyrenoidosa of Algal-Inhibiting Allelochemicals in the Macrophyte, Phragmites communis Tris. |
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| LI Feng min and HU Hong ying.Isolation and Effects on Green Alga Chlorella pyrenoidosa of Algal-Inhibiting Allelochemicals in the Macrophyte, Phragmites communis Tris.[J].Chinese Journal of Environmental Science,2004,25(5):89-92. |
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| Authors: | LI Feng min and HU Hong ying |
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| Institution: | ESPC Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China. |
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| Abstract: | Two fractions of secondary metabolites which inhibited the growth of green alga Chlorella pyrenoidosa, was isolated by several separation techniques from the ethanol extract of the macrophyte, Phragmites communis Tris. The EC50 of the two fractions on C. pyrenoidosa were 0.68mg/L(the fraction of acidic solvent) and 2.65mg/L(the fraction of alkaline solvent), respectively. Further purification of the allelochemicals in the acidic solvent by alkaline aluminum oxide column chromatography revealed that this fraction containing inhibiting allelochemicals and stimulating allelochemicals The inhibiting allelochemicals showed more inhibitory effect and the EC50 was 0.50mg/L. The result suggests that allelochemicals in P. communis can be used as an algae inhibitor. |
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| Keywords: | Chlorella pyrenoidosa Phragmites communis Tris inhibition allelopathy |
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