Tween 20 and Its Major Free Fatty Acids as Carbon Substrates for the Production of Polyhydroxyalkanoates in Pseudomonas aeruginosa ATCC 27853 |
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| Authors: | Giuseppe Impallomeni Salvatore P P Guglielmino Santina Carnazza Annamaria Ferreri Alberto Ballistreri |
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| Institution: | (1) Istituto per la Chimica e la Tecnologia dei Materiali Polimerici, Consiglio Nazionale delle Ricerche, Viale A. Doria 6, 95125 Catania, Italy;(2) Istituto Policattedra di Microbiologia, Sezione MVFA, Facoltà di Scienze, Università di Messina, Sal. Sperone 31, Vill. S. Agata, 98166 Messina, Italy;(3) Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, 95125 Catania, Italy |
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| Abstract: | The ability of Pseudomonas aeruginosa ATCC 27853 to grow and synthesize polyhydroxyalkanoates (PHAs) using Tween 20 as the sole carbon source was investigated. Tween 20 could support cell growth and PHA production. The polymer produced from Tween 20 was compared with those produced from its major free fatty acids components: lauric (C12), myristic (C14), and palmitic (C16) acids. Gas-chromatographic analysis of methanolyzed samples and 13C-Nuclear Magnetic Resonance (NMR) showed that the PHAs obtained are composed of even carbon atoms 3-hydroxyalkanoates ranging from C6 to C14, with C8 and C10 as the predominant components. The nature of the carbon sources used had little influence on the composition, but was found to be important in determining the average molecular weight, shorter chain fatty acids yielding higher molecular weight products. Fast Atom Bombardment-Mass Spectrometry (FAB-MS) of partially pyrolyzed samples, coupled to statistical analysis, showed that these PHAs are random copolymers. |
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| Keywords: | Pseudomonas aeruginosa PHA Tween 20 FAB-Mass Spectrometry |
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