多壁碳纳米管引起白鼠肺部毒性的评价(Comparative Pulmonary Toxic Assessment of Mutil-Wall Carbon Nanotubes in Rats)

以雄性Wistar大白鼠为研究对象,观察了多壁碳纳米管(MWNTs)体内暴露后肺部的病理学变化.采用气管注入方式将直径为40～60nm多壁碳纳米管(40～60MWNTs)和直径小于10nm的多壁碳纳米管(10MWNTs)暴露于大白鼠的体内,同时分别采用纳米二氧化硅(SiO2)和乙炔碳黑(Cb)作为阳性和阴性对照.实验结果表明:实验中采用的纳米颗粒均不同程度地引起了肺部的损伤,同剂量下损伤严重程度顺序为:Cb>10MWNTs>40～60MWNTs>SiO2.多壁碳纳米管对肺部损伤的程度与剂量呈依赖关系,当在低剂量(1mg·kg-1)时,40～60MWNTs仅仅对肺部引起了轻微的炎症损伤,随着剂量的增大,多壁碳纳米管对肺部的损伤越来越严重,出现明显的病理损伤.

Comparative Pulmonary Toxic Assessment of Mutil-Wall Carbon Nanotubes in Rats

Male Wistar rats have been used to study the biological effects of mutil-wall carbon nanotubes(MWNTs).40~60nm MWNTs(40~60MWNTs) and<10nm MWNTs(10MWNTs) were exposed into Wistar rats using intratracheal instillation administration modes.At the same time,silica(SiO2) and acetylene carbon black(Cb) were used as positive and negative control,respectively,to access the toxicity of MWNTs to lung of rats.The results show that the nanoparticles caused different levels of lung damage.The order of lung injury degree followed as Cb>10MWNTs>40~60MWNTs>SiO2.In addition,MWNTs showed dose-dependent relationship on the extent of lung injury.In the low-dose(1mg·kg-1),40~60MWNTs only caused minor damage to lung inflammation.As the dose increased,the lung injury degree and pathological changes became serious.