Electrophoretic identification and genetic analysis of bivalve larvae |
| |
| Authors: | Ya-Ping Hu Richard A Lutz Robert C Vrijenhoek |
| |
| Institution: | (1) Institute of Marine and Coastal Sciences, Rutgers University, 08903 New Brunswick, New Jersey, USA;(2) Center for Theoretical and Applied Genetics, Rutgers University, 08903 New Brunswick, New Jersey, USA;(3) the Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, USA |
| |
| Abstract: | Taxonomic identification and genetic analysis of larval marine invertebrates have been vexing problems. We describe a polyacrylamide mini-gel electrophoresis technique for resolving proteins from individual larval bivalves (shell length 250 to 350  m) and apply this technique to three species of laboratory-cultured larval oysters Ostrea edulis L., 1758, Crassostrea gigas (Thunberg, 1793) and c. virginica (Gmelin, 1791)] reared during summer 1989. Electrophoretic patterns of proteins clearly discriminate among the three species and allow genetic analysis of a polymorphic allozyme locus (Pgi) in field-collected larvae and juveniles of C. virginica. This technique provides an economical tool for largescale taxonomic, ecologic, and genetic studies of meroplanktonic stages of various species. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
