城镇污泥中神经酰胺的分离纯化

以A²O-MBR工艺的冷冻干燥污泥作为原料，以索氏提取法，获得污泥中的粗脂；再经硅胶柱层析，以石油醚-乙酸乙酯100：1，10：1和7：3（V/V）的顺序梯度洗脱，利用薄层色谱法，将洗脱液与神经酰胺标准品对比，V（石油醚）：V（乙酸乙酯）=7：3为合适的洗脱梯度.经Q-Exactive液相色谱-高分辨质谱检测各组成成分，就峰面积比，经柱层析洗脱前后神经酰胺从60.88%提高到98.34%.最后，用另一种A²O工艺的污泥进行尝试，柱层析前后神经酰胺从79.06%提高至94.15%.

Preliminary study on extraction and purification of ceramide in sewage sludge

Using the freeze-dried sludge of A²O-MBR process as raw material, we firstly obtained the crude lipid in the sludge by Soxhlet extraction. Then the crude lipid was eluted in a sequence of petroleum ether-ethyl acetate 100:1, 10:1 and 7:3 (V/V) by silica gel column chromatography. Compared the eluent with ceramide standard by thin layer chromatography, it was proved that the appropriate elution gradient was petroleum ether-ethyl acetate 7:3 (V/V). Through Q Exactive bench quadrupole orbitrap high-resolution mass spectrometry, the peak area ratio of ceramide was increased from 60.88% to 98.349% in the process of column chromatography. When the sludge of A²O process was used as raw material in the same experiment, the peak area ratio of ceramide was increased from 79.06% to 94.15%.