一株高适应性Nitrosomonas eutropha CZ-4的脱氨特性

从垃圾渗滤液中分离得到了一株亚硝化单胞菌Nitrosomonas eutropha CZ-4，其16S rDNA序列与N.eutropha C91的相似性达99%.研究了pH值、温度、游离亚硝酸浓度、盐度等对其生长的影响，并测试了其在垃圾渗滤液、黑臭水和富营养化湖水中的脱氨效果.结果表明，该菌的最适生长pH值为7.3~8.7，最适生长温度为30.9℃，游离亚硝酸和盐度对该菌的半数抑制浓度分别约为0.11mg/L与2%.在最佳发酵条件下，该菌的最大氨氮去除速率为58mg/（L·h），最短倍增时间为8.2h；在不同类型的污水/地表水（初始氨氮浓度为0.66~603mg/L）中，该菌的最大氨氮去除速率为11.4mg/（L·h），最短倍增时间为10.9h，最低残余氨氮浓度为0.11mg/L.

Nitrogen removal characteristics of a highly adaptable Nitrosomonas eutropha CZ-4

A Nitrosomonas strain was isolated from the landfill leachate. It was named as Nitrosomonas eutropha CZ-4 for its 16s rDNA sequence was highly similar to N. eutropha C91at an identity of 99%. This work studied the influences of pH, temperature, free nitrous acid concentration and salinity on the growth of N. eutropha CZ-4, as well as its NH₃-N removal abilities in landfill leachate, black odor water and eutrophic lake water. The results showed that the optimum growth pH of the strain was 7.3~8.7, the optimum growth temperature was 30.9℃, and the IC₅₀ of free nitrous acid and salinity was about 0.11mg/L and 2% (in terms of NaCl), respectively. Under the optimal fermentation condition, the maximum NH₃-N removal rate reached 58mg/(L·h), and the shortest doubling time was 8.2h. In different types of sewage/surface water (their initial ammonia concentration ranged from 0.66mg/L to 603mg/L), the NH₃-N removal rate was up to 11.4mg/(L·h), the doubling time was as short as 10.9h, and the residual NH₃-N concentration was down to 0.11mg/L.