倏逝波光纤传感器快速检测诺氟沙星

基于间接竞争免疫分析原理,利用倏逝波光纤生物传感平台研发了一种诺氟沙星检测方法,实现了水中诺氟沙星的快速､灵敏检测.研究表明,诺氟沙星检测的优化条件为:抗体浓度为1μg/mL､预反应时间为1min,反应时间为4min.优化条件下,诺氟沙星检测限可达1.89μg/L.包被抗原修饰的光纤探头与荧光标记诺氟沙星抗体具有良好的特异性和稳定性,可重复使用400次以上.自来水､景观水､二沉池出水等水样的加标回收实验结果表明,该传感器具有良好的精密度和准确性,受环境基质的影响较小,能够用于实际水样中诺氟沙星的快速检测.

Rapid detection of norfloxacin in water by evanescent wave fiber optic biosensor

Norfloxacin (NOR), a widely used antibiotic, is becoming a major threat to ecological security. The existing detection methods have either high cost and pretreatment requirements or long detection time and poor sensitivity. Based on indirect competitive immunoassay principle, a new detection method of norfloxacin was developed using the evanescent wave fiber optic bio sensing platform. The rapid and sensitive detection of norfloxacin in water was achieved. The optimal detection conditions of norfloxacin were as follows: antibody concentration was 1μg/mL, pre-reaction time was 1min, and reaction time was 4min. Under the optimal conditions, the detection limit of norfloxacin was 1.89μg/L. The coating-antigen modified fiber optic probe showed good specificity for fluorescence-labeled anti-norfloxacin antibody and stability, and could be reused more than 400 times. The recovery results of the spiked water samples, such as tap water, landscape water, and effluent of secondary sedimentation tank, demonstrated that the proposed biosensor had good precision and accuracy, and was less affected by the environmental matrix. The presented biosensor can be used for the rapid detection of NOR in actual water samples.