MBBR工艺中SNEDPR的启动及性能研究

为探究同步硝化内源反硝化除磷(SNEDPR)强化移动床生物膜反应器(MBBR)工艺脱氮除磷的可行性,采用连续曝气和搅拌/曝气交替运行的MBBR反应器,以磁性填料作为载体处理模拟生活污水,考察了SNEDPR启动过程中的脱氮除磷性能,并结合荧光显微镜和高通量测序技术对各个功能菌群结构变化情况进行了分析.结果表明,经两阶段运行后,氨氮和磷去除率分别达到97.6%和85.37%,出水NO₂^-—N、NO₃^-—N和COD浓度分别为1.3949,3.88和20.4mg/L,同步硝化内源反硝化率(SNEDR)由0.07%逐渐升高至86.35%.好氧阶段同步硝化内源反硝化率的提高,使出水NO_x^-—N浓度下降,提高了系统的脱氮性能和厌氧阶段内碳源的储存量.荧光显微镜和高通量测序结果表明,经过53d的运行,微生物群落多样性呈显著提高,系统内GAOs、AOB、NOB丰度的提高(分别由接种污泥中的3.3%、0.84%和0.66%提高至系统内的27.08%/20.48%、1.45%/1.76%和1.05%/0.85%)和PAOs、DPAOs的存在,保证了系统的脱氮除磷性能,在MBBR工艺中实现了EBPR与SNED的耦合.

Start-up and performance study on the simultaneous nitrification-endogenous denitrification phosphorus removal (SNEDPR) in the biological process of the moving bed biofilm reactor (MBBR)

This study assessed the feasibility of simultaneous nitrification, endogenous denitrification and phosphorus removal (SNEDPR) for enhancing the nitrogen and phosphorus removal by the process of moving bed biofilm reactor (MBBR), an MBBR operated alternately by continuous aeration and stirring/aeration was adopted for simulating the treatment of domestic sewage with magnetic filler as the carrier. The performance of nitrogen and phosphorus removal during SNEDPR initiation was investigated, and the structural changes of functional microflorae were analyzed by fluorescence microscopy and high-throughput sequencing technology. The results showed that after two stages of operation, the removal rates of ammonia nitrogen and phosphorus reached 97.6% and 85.37% respectively, and the NO₂^--N, NO₃^--N and COD concentrations in the effluent were 1.3949, 3.88 and 20.4mg/L respectively. The simultaneous nitrification and endogenous denitrification rate (SNEDR) increased from 0.07% to 86.35%. The improvement in SNEDR at the aerobic stage decreased the NO_x^--N concentration in the effluent, improved the denitrification performance of the system and promoted the storage capacity of carbon source at the anaerobic stage. The fluorescence microscopy and high-throughput sequencing results showed that after 53d of operation, the diversity of microbial community was improved significantly, and at the same time, the increase in abundance for GAOs, AOB and NOB (from 3.3%, 0.84% and 0.66% in the inoculated sludge to 27.08%/20.48%, 1.45%/1.76% and 1.05%/0.85% in the system respectively) and the presence of PAOs and DPAOs guaranteed the performance of nitrogen and phosphorus removal for the system, and realized the coupling of EBPR and SNED by the MBBR process.