污泥絮体特征与微型动物运动速度相关性研究——以自由运动型微型动物为例

为了克服利用微型动物群落物种变化指示活性污泥运行状态存在的微型动物鉴别误判、分类和统计分析工作量大等问题，通过PCA法的相关性分析判断絮体微观形态参数降维成综合指标的可能性，采用“微型动物运动视频采集+定量图像分析”法表征微型动物的运动速度（平均线速度V和平均角速度W），分析SBR系统中锐利楯纤虫（A.lynceus）等7种自由运动型微型动物的运动速度与污泥絮体微观特性之间的相关性.结果表明：多个污泥絮体微观形态参数之间存在较好的相关性，可降维成2大综合指标（絮体大小密实度（SC）和絮体形状规则度（SR））.絮体SC和SR指标与须足轮虫属（Euchlanis sp.）、凹扁前口虫（F.depressa）和锐利楯纤虫（A.lynceus）的运动速度之间具有较显著相关性（|r|>0.50，P<0.05），因此利用典型的自由运动型微型动物运动速度变化能够快速指示污泥絮体的微观形态变化.

Microscopic characteristics of sludge flocs as related to the movement velocity of microfauna: A case study of free-moving microfauna

This study was designed to figure out the problems such as misjudgment of micro-animals, heavy workload of classification and statistical analysis, etc., which exist in using changes in micro-animal community species to indicate the operating status of activated sludge. The possibility of reducing the dimensions of floc micromorphological parameters into a comprehensive index was judged with the principal component analysis (PCA). Meanwhile, the "micro-animal movement video acquisition + quantitative image analysis" method was used to characterize the movement speed of micro-animals(average linear velocity V and average angular velocity W) and identify the correlations between the movement speeds of seven freely moving micro-animals such as A. lynceus and the microscopic characteristics of sludge flocs in the SBR system. The results show a good correlation between the microscopic morphological parameters of the sludge flocs, which can be reduced to two comprehensive indicators (floc size and compactness (SC) and floc shape regularity (SR)). Both the floc SC and SR indicators were observed to have a significant correlation to the movement speed of Euchlanis sp., F. depressa and A. lynceus (|r|>0.50, P<0.05), so the change in the movement speed of typical free-moving micro-animals can quickly indicate the micro-morphological changes in sludge flocs.