内碳源短程反硝化启动及EPD-ANAMMOX耦合工艺性能

接种普通活性污泥,以乙酸盐为碳源,控制进水COD/P为150∶1,在A/O SBR反应器内富集培养了聚糖菌;采用逐渐提高SBR厌氧末硝酸盐投加浓度的方法,将聚糖菌驯化诱导为反硝化聚糖菌结果.SBR厌氧末排水中COD与缺氧末排水基本相同,COD平均去除率达到86.74％,总氮去除率达到98％以上.然后缩短SBR的厌氧及缺...

Start-up of endogenous partial denitrification and performance of EPD-ANAMMOX coupling process

This study was designed to evaluate performances of the start-up of endogenous partial denitrification and EPD-ANAMMOX process. The glycogen accumulation bacteria (GAO) were enriched in the anaerobic/aerobic sequence batch reactor (SBR) using the activated sludge as inoculum sludge and the acetate acted as carbon source by regulating the influent COD/P ratio as 150:1. Then, the GAOs were induced into denitrifying glycogen accumulation bacteria (DGAO) by means of gradually boosting the concentration of added nitrate at the end of anaerobic phase in SBR. Correspondingly, the level of chemical oxygen demand (COD) at the end of anoxic period was almost the same as that at the distal end of anaerobic stage, the total nitrogen removal efficiency and the average COD removal efficiency were over 98% and 86.74% at the end of anaerobic period of SBR, respectively. Furthermore, the endogenous partial denitrification system was successfully formed by shortening the anaerobic and anoxic time, the ratio of nitrite translated from nitrate (NTR) at the end of anoxic period was up to 65.96%. The average total nitrogen and COD removal load in the 30days operation of the coupled process were 0.222kgN/(m³×d) and 0.337kgCOD/(kgMLVSS.d) with an efficiency of 86.12% and 87.21%, respectively. Typically, the effluent NO₃^--N concentration was lower than 4.2mg/L and both NO₂^--N and NH₄⁺-N levels were near to 0mg/L in the same phase. Compared with inoculated sludge, the relative abundance of competibacter increased from 0.001% to 25.06% in the sludge of EPD-SBR during the stable operation period, and the total abundance of Defluviicoccus, Contendobacter, Sphingobium,and Amaricoccus increased from 0.14% to 0.431%, implying that the competibacter was the dominated functional bacteria for the endogenous partial denitrification system.