大鼠不同脏器亚硫酸氧化酶活性及其与年龄的关系

为了探讨体内亚硫酸氧化酶的分布特点及随年龄的变化规律,以Wistar大鼠为实验材料,研究了不同月龄大鼠脏器中的亚硫酸氧化酶活性.以细胞色素C为电子受体,应用分光光度法测定了不同月龄(1、4、10个月)大鼠肝、肾、肺、脾、脑、胃及胸主动脉中亚硫酸氧化酶的活性.结果表明:1)亚硫酸氧化酶在大鼠全身主要脏器中普遍存在,且在不同脏器中该酶的活性不同,从强到弱的顺序基本表现为:肝、肾>胃>脑>胸主动脉血管>肺>脾;2)肝、肾、血管组织中亚硫酸氧化酶活性与大鼠年龄相关,肝脏亚硫酸氧化酶活性随年龄的增加而增加(Spearman,r=0.674,p<0.001),而肾脏和胸主动脉血管该酶活性随年龄的增加而减小(Spearman,r=-0.756,p<0.001;r=-0.629;p<0.05),其余脏器未见明显变化;3)亚硫酸氧化酶活性降低可能是一种机体衰老的生化标志物.

Sulfite Oxidase Activity in Various Organs of Different Aged Rats

To study the activity of sulfite oxidase in different organs and the relation between its activity and age, the activities of sulfite oxidase in liver, kidney, lung, spleen, brain, stomach and thoracic aorta in different aged(1 month, 4 month and 10 month)Wistar rats were assayed. Results indicated that:1)Sulfite oxidase was found in most tissues of the body, and its activity was different in different organs and tissues. The sequence of sulfite oxidase activity from high to low was:liver, kidney>stomach>brain>thoracic aorta>lung>spleen;2)Sulfite oxidase activities in liver, kidney and thoracic aorta were related to the age of rats. Sulfite oxidase activity in liver was positively related to age(Spearman, r=0.674, p<0.001), and negatively related in kidney and thoracic aorta(Spearman, r=-0.756, p<0.001;r=-0.629, p<0.001). The other tissues were not significantly related to age;3)The decrease of sulfite oxidase activity might be a biomarker of aging.