PCR-RFLP技术在环境地球化学研究中的应用及展望

PCR—RFLP技术为环境地球化学提供了一种新的研究方法和实验设计的新的思维方式。该方法具有所需样品量低、快速简便及特征性强等优点 ,可广泛应用于物质的生物地球化学循环、环境过程的生物作用、生物多样性及有机物源判断等方面的研究。利用PCR—RFLP技术 ,以环境中存在的 16SrRNA为对象 ,对环境生物的研究已广泛开展并取得了许多成果。展望未来研究成果 ,将会在海洋及湖泊沉积物等自然环境中发现更多的、新的微生物种类 ;将进一步阐明生物作用和物质循环的机理和过程 ;进一步阐明自然环境中生物大分子的变化机理及其环境效应并找到判断沉积物有机物源的新方法

EXPECTATION AND APPLICATION OF PCR-FFLP IN ENVIRONMENENTAL GEOCHEMISTRY

Application of PCR-RFLP technique in environmental geochemistry provides a new research method, as well as a manner of thinking in experimental design. The method has many advantages such as low sample requirement, faster performance and convenience. It can be widely applied in the study of biogeochemical cycle of substances, biological effects in environmental processes, biological diversity and identification of the sources of organic substances. By using the PCR—RFLP technique, environmental biology, which takes 16S rRNA in the environment as research object, has been studied and many results have been achieved. Prospects of the future research are: more and unknown microorganisms will be discovered in natural environments such as oceans and lakes, ulterior mechanism and processes of biological effects and substantial cycle and ulterior change mechanism of biological macromolecules in natural environments will be enucleated, and a new method to identify the origin of organic substances.