| 好氧反硝化菌的化学-物理法诱变育种研究 |
| |
| 引用本文： | 李永智,汪苹,廖小红,刁惠芳.好氧反硝化菌的化学-物理法诱变育种研究[J].环境科学与技术,2010,33(3). |
| |
| 作者姓名： | 李永智 汪苹 廖小红 刁惠芳 |
| |
| 作者单位： | 1. 北京工商大学化学与环境工程学院,北京,100037
2. 美国哈希公司北京代表处,北京,100004 |
| |
| 基金项目： | 国家"十一五"科技支撑计划课题 |
| |
| 摘 要： | 以实验室SBR反应器分离出的好氧反硝化菌(WXZ-17)为原始菌株,以硫酸二乙酯(DES)为诱变剂进行化学诱变。通过反硝化性能测定筛选较好的正突变体1号,其NO3--N还原率比原始菌株WXZ-17高20.33%,做为物理诱变的出发菌株以紫外线为诱变剂进行物理诱变,优选出的1～6号的NO3--N还原率达到56.11%,比原始菌株WXZ-17号的NO3--N还原率高24.4%,且NO2--N基本没有积累。通过化学—物理相结合的复合诱变法,成功有效地改良了WXZ-17号菌株。
|
| 关 键 词： | 好氧反硝化菌 化学诱变 紫外线诱变 |
Mutation Breeding with Chemi-physical Methods by Aerobic Denitrifiers |
| |
| LI Yong-zhi,WANG Ping,LIAO Xiao-hong,DIAO Hui-fang.Mutation Breeding with Chemi-physical Methods by Aerobic Denitrifiers[J].Environmental Science and Technology,2010,33(3). |
| |
| Authors: | LI Yong-zhi WANG Ping LIAO Xiao-hong DIAO Hui-fang |
| |
| Institution: | LI Yong-zhi1,WANG Ping2,LIAO Xiao-hong3,DIAO Hui-fang4 (1.School of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100037,China,2.Beijing Representative Office,American Hach Company,Beijing 100004,China) |
| |
| Abstract: | Selecting aerobic denitrification strain (WXZ-17) isolated from SBR reactor in the laboratory as the original strain, chemical mutation was done with DES as mutagen. Screening the best mutant No. 1 as the original strain of physical mutation through measured denitrification ability of mutants, its reduction rate of NO_3~--N was 20.33% higher than the original strain. Mutating with UV as mutagen, the reduction rate of NO_3~--N of the strain No. 1～6 selected was 24.4% higher than the original strain WXZ-17 with NO_3~--N accumulation. Aerobic denitrification ability of WXZ-17 was effectively improved through chemi-physical mutation. |
| |
| Keywords: | aerobic denitrifiers chemical mutation UV-ray mutation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
