| 农业废物好氧堆肥过程因子对细菌群落结构的影响 |
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| 引用本文： | 张嘉超,曾光明,喻曼,陈耀宁,黄红丽,李辉,余震,彭亿. 农业废物好氧堆肥过程因子对细菌群落结构的影响[J]. 环境科学学报, 2010, 30(5): 1002-1010 |
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| 作者姓名： | 张嘉超 曾光明 喻曼 陈耀宁 黄红丽 李辉 余震 彭亿 |
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| 作者单位： | 湖南大学环境科学与工程学院,长沙,410082;湖南大学环境生物与控制教育部重点实验室,长沙,410082;浙江省农业科学院环境资源与土壤肥料研究所,杭州,310021;湖南农业大学资源环境学院,长沙,410128 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(No.50808072, 50608029);国家杰出青年科学基金(No.50425927);国家重点基础研究发展计划(973)项目(No.2005CB724203);国家教育部长江学者和创新团队发展计划项目(No.IRT 0719) |
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| 摘 要： | 采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术研究了农业废物堆肥过程中细菌种群随时间的变化.同时,应用Quantity One2.0和Canoco4.5软件对获得的堆肥细菌种群数据与堆肥过程因子:环境温度、堆体温度、pH、含水率、水溶性有机碳(WSC)、C/N、水溶性氨氮(NH4+-N)和硝氮(NO3--N)进行冗余分析,并做出样点、种群与堆肥过程因子的二维排序图.结果表明,细菌群落(样点)以堆肥过程因子为梯度大体可划分为升温期(1～2d)、高温期(3～11d)、降温期(12～18d)和腐熟期(19～36d)4个阶段,每一阶段均有对应种群存在.不同的堆肥过程因子对细菌种群的影响大小依次为:NO3--N堆体温度WSCC/NNH4+-N含水率pH环境温度,其中,堆体温度、WSC、NO3--N、NH4+-N对细菌种群的影响极显著(p0.01),C/N、pH对细菌种群的影响显著(p0.05),含水率、环境温度对细菌种群的影响不显著.
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| 关 键 词： | 堆肥 细菌群落 变性梯度凝胶电泳(DGGE) 冗余分析 |
| 收稿时间： | 2009-08-30 |
| 修稿时间： | 2009-12-14 |
Succession of bacterial community structure in agricultural waste composting under different conditions |
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| ZHANG Jiachao,ZENG Guangming,YU Man,CHEN Yaoning,HUANG Hongli,LI Hui,YU Zhen and PENG Yi. Succession of bacterial community structure in agricultural waste composting under different conditions[J]. Acta Scientiae Circumstantiae, 2010, 30(5): 1002-1010 |
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| Authors: | ZHANG Jiachao ZENG Guangming YU Man CHEN Yaoning HUANG Hongli LI Hui YU Zhen PENG Yi |
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| Affiliation: | 1. College of Environmental Science and Engineering, Hunan University, Changsha 410082; 2. Key Laboratory of Environmental B iology and Pollution Control(Hunan University), Ministry of Education, Changsha 410082,1. College of Environmental Science and Engineering, Hunan University, Changsha 410082; 2. Key Laboratory of Environmental B iology and Pollution Control(Hunan University), Ministry of Education, Changsha 410082,Environmental Resources and Soil Fertilizer Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,1. College of Environmental Science and Engineering, Hunan University, Changsha 410082; 2. Key Laboratory of Environmental B iology and Pollution Control(Hunan University), Ministry of Education, Changsha 410082,College of Resources and Environment, Hunan Agricultural University, Changsha 410128,College of Environmental Science and Engineering, Hunan University, Changsha 410082; 2. 2. Key Laboratory of Environmental B iology and Pollution Control(Hunan University), Ministry of Education, Changsha 410082,College of Environmental Science and Engineering, Hunan University, Changsha 410082; 2. 2. Key Laboratory of Environmental B iology and Pollution Control(Hunan University), Ministry of Education, Changsha 410082 and College of Environmental Science and Engineering, Hunan University, Changsha 410082; 2. 2. Key Laboratory of Environmental B iology and Pollution Control(Hunan University), Ministry of Education, Changsha 410082 |
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| Abstract: | |
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| Keywords: | composting bacterial community denaturing gradient gel electrophoresis (DGGE) redundancy analysis (RDA) |
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