高内涵筛选技术的原理及其在生态毒理学的应用

大量存在于环境中的有毒污染物仍然缺乏足够的毒理学数据来对其进行有效的监管,为了满足海量化合物毒性评价的需要,基于离体生物测试的高通量毒性筛选方法在近些年得到了迅猛的发展。高内涵筛选技术是新型的高通量化合物毒性筛选方法,该方法最显著的特点是能够在保持细胞结构和功能完整的基础上同时获取多种毒性指标。因此在简介高内涵筛选技术原理的基础上,综述了其在生态毒理学领域已有的应用,并针对性地对高内涵筛选技术的发展和挑战进行了展望。

Principles and Applications of High Content Screening Technology in Ecotoxicology

As a large amount of environmental pollutants may pose hazardous effects on the environment and human beings at low concentrations, evaluating the toxic effects of these chemicals and understanding their mechanisms of action (MOA) are essential for management of chemical environmental risks. Therefore, high-throughput in vitro bioassay methods have been rapidly developed to satisfy the toxicity assessment of countless chemicals. High-content screening (HCS), as a new cell-based screening approach, allows multi-endpoint determination simultaneously in intact cells. This review introduced the principles of HCS and summarized its application in ecotoxicology. Finally, the new developments and challenges of HCS are proposed.