Determination of canthaxanthin in the red coral (Corallium rubrum) from Marseille by HPLC combined with UV and MS detection |
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| Authors: | Jelena Cvejic Sylvie Tambutté Severine Lotto Momir Mikov Ivan Slacanin Denis Allemand |
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| Affiliation: | (1) Faculty of Medicine, Department of Pharmacy, Laboratory for Pharmaceutical and Natural Products Analysis, Hajduk Veljkova 3, 21000 Novi Sad, Serbia;(2) Centre Scientifique de Monaco, Av St Martin, Monaco, MC, 98000, Monaco;(3) School of Pharmacy, University of Otago, Dunedin, New Zealand;(4) ILIS, Ch. De la Passerelle 17, Bienne, Switzerland |
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| Abstract: | Corallium rubrum is the most famous and precious coral due to the intense characteristic red colour of its skeleton. We have determined the presence of carotenoids in natural samples of C. rubrum from Marseille, Riou, and investigated their chemical composition. Analysis was performed both on soft tissues and hard tissues including spicules and skeleton. Since hard tissues are made of a mineral fraction and an organic fraction obtained after demineralization, extraction was performed both with and without demineralization by EDTA. The extracts were analyzed by complementary methods of TLC, HPLC/DAD and HPLC/MS. The components were separated by RP-18 chromatography column using acetonitrile/methanol for HPLC/DAD and acetonitrile/water gradient for HPLC/MS analysis. Our results give the first evidence of the presence of canthaxanthin, 4,4′-diketo-β-carotene, as the major carotenoid in all samples. Spicules exhibited higher content in carotenoids than skeleton. Demineralization treatment improved the efficiency of carotenoid extraction by an average factor of 5 and showed that organic matrix contains canthaxanthin. |
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