Influence of Carbon Sources on The Production and Characterization of The Exopolysaccharide (EPS) by Bacillus sphaericus 7055 Strain |
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| Authors: | Murat Yilmaz Gokcen Yuvali Celik Belma Aslim Dilsad Onbasili |
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| Institution: | (1) Department of Biology, Faculty of Science and Arts, Nigde University, Campus, 51200 Nigde, Turkey;(2) Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey;(3) Molecular Biology Research Center, Gazi University, 06830 Ankara, Turkey; |
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| Abstract: | In this study, EPS produced by five Bacillus spp. strains was determined. The only one strain (B. sphaericus 7055) was selected due to its high EPS production and it was investigated by growing this strain in LB broth medium containing
various carbon sources. The highest EPS production of this strain was found in medium containing fructose. However, the effect
of different concentrations of fructose and molasses on EPS production by the strain was studied. The maximum EPS yield of
the strain 7055 was recorded with 2.5% (w/v) fructose, also the highest EPS production was found in 2.5% (w/v) molasses. The
strain 7055 was found to contain (98.6%) galactose and (1.4%) glucuronic acid in control medium whereas the composition of
the strain 7055 (2.5% (w/v) fructose) was found to be (99.9%) neutral sugar and (0.1%) Glucuronic acid while the strain 7055
(2.5% (w/v) molasses) was found to contain (65.9%), neutral sugars and (34.1%) glucuronic acid. |
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