| 一种简便快速检测涕灭威对DNA损伤的方法 |
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| 引用本文： | 张清敏,熊瑛,李得翔,戴树桂.一种简便快速检测涕灭威对DNA损伤的方法[J].环境科学,2001,22(5):126-128. |
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| 作者姓名： | 张清敏 熊瑛 李得翔 戴树桂 |
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| 作者单位： | 南开大学环境科学与工程学院,天津,300071 |
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| 基金项目： | 国家自然科学基金资助重点项目(29837170) |
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| 摘 要： | 介绍一种简便、快速检测涕灭威对DNA损伤的方法,并将这种方法与其他几种检测DNA损伤的方法进行了比较.这种方法直接把菌体或组织匀浆液放入琼脂糖凝胶的加样孔穴中,在原位裂解后,在较高pH环境下进行电泳,根据DNA的量和损伤程度评价涕灭威对生物个体或生态系统产生的影响.
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| 关 键 词： | DNA损伤 电泳 图像分析 |
| 文章编号： | 0250-3301(2001)05-03-0126 |
| 收稿时间： | 2000/11/2 0:00:00 |
| 修稿时间： | 2000年11月2日 |
A Simple and Rapid Procedure for Detection of DNA Damage Caused by Aldicarb |
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| Zhang Qingmin,Xiong Ying,Li Dexiang and Dai Shugui.A Simple and Rapid Procedure for Detection of DNA Damage Caused by Aldicarb[J].Chinese Journal of Environmental Science,2001,22(5):126-128. |
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| Authors: | Zhang Qingmin Xiong Ying Li Dexiang and Dai Shugui |
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| Institution: | College of Environmental Science and Engineering, Nankai University, Tianjin 300071. |
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| Abstract: | A simple and rapid procedure for detection of low levels DNA damage caused by Aldicarb was developed and compared with some other methods. In the original position the gel was set in high pH buffer to run electrophoresis after the diluent of bacteria or fragmentized organ was added into hole of agarose gel directly and cells were treated with detergent and high alkaline condition. The impact of Aldicarb to biological individual or ecosystem was assessed according to the quantity of DNA and the extent of DNA migration. |
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| Keywords: | DNA damage electrophoresis image analysis |
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